Calcium uptake by casein embedded in polyelectrolyte multilayer

被引：13

作者：

Szyk-Warszynska, Lilianna ^{[1
]}

Gergely, Csilla ^{[2
]}

Jarek, Ewelina ^{[1
]}

Cuisinier, Frederic ^{[3
]}

Socha, Robert P. ^{[1
]}

Warszynski, Piotr ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Catalysis & Surface Chem, PL-30239 Krakow, Poland

[2] Univ Montpellier 2, CNRS, UMR 5650, Etud Semicond Grp, F-34095 Montpellier 05, France

[3] Univ Montpellier I, UFR Odontol, F-34193 Montpellier, France

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2009年 / 343卷 / 1-3期

关键词：

Casein; Layer-by-layer; Polyelectrolyte films; Calcium binding; Multilayers; ADSORPTION; MICELLES; PROTEINS; FILMS;

D O I：

10.1016/j.colsurfa.2009.01.038

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The aim of our work was to investigate formation of polyelectrolyte multilayer films containing alpha- and beta-casein. Since in neutral pH casein is negatively charged (as verified by electrophoretic mobility measurements) it has been used as a polyanionic layer for the film build-up. Casein containing films were formed on Si/SiO2 wafers. The formation of the film was investigated by liquid cell ellipsometry. After the multilayer films were formed they were contacted with solution containing calcium ions and changes in the film thickness were monitored. Additionally the Surfaces of casein containing multilayers were analyzed with AFM for the structural changes within the films occurring after binding of calcium ions. Presence of calcium ions bound in the film was also monitored by XPS. We concluded that casein embedded in the polyelectrolyte multilayers preserves its ability to bind calcium ions. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：118 / 126

页数：9

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