Effects of nitric oxide, light adaptation and APB on spectral characteristics of H1 horizontal cells in carp retina

被引:11
|
作者
Yamada, M
Fraser, SP
Furukawa, T
Hirasawa, H
Katano, K
Djamgoz, MBA
Yasui, S
机构
[1] Electrotech Lab, Supermol Div, Tsukuba, Ibaraki 305, Japan
[2] Univ London Imperial Coll Sci Technol & Med, Dept Biol, Neurobiol Grp, London SW7 2AZ, England
[3] Kyushu Inst Technol, Iizuka, Fukuoka 820, Japan
关键词
retina; horizontal cell; nitric oxide; 2-amino-4-phosphonobutyrate; light adaptation; dark adaptation; colour vision; dopamine;
D O I
10.1016/S0168-0102(99)00094-2
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The spectral characteristics of cone-driven horizontal cells of H1 subtype (H1 HCs) receiving main synaptic input from red-sensitive cones were studied in light- and dark-adapted retinae of carp. The spectral sensitivity profile of H1 HCs in dark-adapted retinae was practically the same as the absorption spectrum of red-sensitive cones. Light-adaptation decreased the sensitivity preferentially in the short-wavelength (blue/green) region, resulting in a relative enhancement of the 617 nm peak. Application of nitric oxide (NO) donors, sodium nitroprusside (SNP) and S-nitrosoglutathione (SNOG or GSNO), or dopamine to dark-adapted retinae decreased the sensitivity preferentially in blue/green region, an effect similar to that of light-adaptation. Application of haemoglobin (Hb, an NO scavenger) or 2-amino-4-phosphonobutyrate (APB, a metabotropic glutamate receptor agonist), to light-adapted retinae increased the sensitivity preferentially in the blue/green region, an effect similar to dark-adaptation. The photoresponses of H1 HCs were univariant in dark-adapted retinae as well as Hb-treated light-adapted retinae. In light-adapted retinae with normal Ringer, however, the univariance did not hold. These results suggested that the photoresponses of H1 HCs to short-wavelength stimuli contain a depolarising (sign-reversing) component, which can be activated by light-adaptation or application of NO and dopamine, and inactivated by dark-adaptation or deprivation of NO or application of APE. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
引用
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页码:309 / 319
页数:11
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