Automatic reactor for solid-phase synthesis of molecularly imprinted polymeric nanoparticles (MIP NPs) in water

被引：86
|
作者：
Poma, Alessandro [1 ]
Guerreiro, Antonio [2 ]
Caygill, Sarah [2 ]
Moczko, Ewa [2 ]
Piletsky, Sergey [2 ]
机构：
[1] Open Univ, Dept Life Hlth & Chem Sci, Milton Keynes MK7 6AA, Bucks, England
[2] Univ Leicester, Dept Chem, Coll Sci & Engn, Leicester LE1 7RH, Leics, England
来源：
RSC ADVANCES | 2014年 / 4卷 / 08期
基金：
英国惠康基金;
关键词：
ANTIBODIES; NANOMEDICINE; IMMUNOASSAYS; STABILITY;
D O I：
10.1039/c3ra46838k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report the development of an automated chemical reactor for solid-phase synthesis of MIP NPs in water. Operational parameters are under computer control, requiring minimal operator intervention. In this study, "ready for use" MIP NPs with sub-nanomolar affinity are prepared against pepsin A, trypsin and a-amylase in only 4 hours.
引用
收藏
页码：4203 / 4206
页数：4
相关论文
共 50 条
  • [1] Solid-phase synthesis of molecularly imprinted nanoparticles
    Canfarotta, Francesco
    Poma, Alessandro
    Guerreiro, Antonio
    Piletsky, Sergey
    NATURE PROTOCOLS, 2016, 11 (03) : 443 - 455
  • [2] Solid-phase synthesis of molecularly imprinted nanoparticles
    Francesco Canfarotta
    Alessandro Poma
    Antonio Guerreiro
    Sergey Piletsky
    Nature Protocols, 2016, 11 : 443 - 455
  • [3] Nucleoside-Tailored Molecularly Imprinted Polymeric Nanoparticles (MIP NPs)
    Poma, Alessandro
    Brahmbhatt, Heli
    Watts, Jonathan K.
    Turner, Nicholas W.
    MACROMOLECULES, 2014, 47 (18) : 6322 - 6330
  • [4] Molecularly Imprinted Nanoparticles (NanoMIPs) Selective for Proteins: Optimization of a Protocol for Solid-Phase Synthesis Using Automatic Chemical Reactor
    Caceres, Cesar
    Moczko, Ewa
    Basozabal, Itsaso
    Guerreiro, Antonio
    Piletsky, Sergey
    POLYMERS, 2021, 13 (03) : 1 - 11
  • [5] Solid-phase synthesis of molecularly imprinted nanoparticles for protein recognition
    Ambrosini, Serena
    Beyazit, Selim
    Haupt, Karsten
    Bui, Bernadette Tse Sum
    CHEMICAL COMMUNICATIONS, 2013, 49 (60) : 6746 - 6748
  • [6] Molecularly imprinted polymeric fibers for solid-phase microextraction
    Turiel, E.
    Tadeo, J. L.
    Martin-Esteban, A.
    ANALYTICAL CHEMISTRY, 2007, 79 (08) : 3099 - 3104
  • [7] Guide to the Preparation of Molecularly Imprinted Polymer Nanoparticles for Protein Recognition by Solid-Phase Synthesis
    Xu, Jingjing
    Medina-Rangel, Paulina X.
    Haupt, Karsten
    Bui, Bernadette Tse Sum
    NANOARMORING OF ENZYMES: RATIONAL DESIGN OF POLYMER-WRAPPED ENZYMES, 2017, 590 : 115 - 141
  • [8] Synthesis and characterization of a molecularly imprinted polymer (MIP) for solid-phase extraction of the antidiabetic gliclazide from human plasma
    Vasconcelos, Ingrid
    Reis da Silva, Pedro Henrique
    Davila Dias, Derick Rodrigues
    de Freitas Marques, Maria Betania
    Mussel, Wagner da Nova
    Pedrosa, Tercio Assuncao
    Ribeiro e Silva, Maria Elisa Scarpelli
    de Souza Freitas, Roberto Fernando
    de Sousa, Ricardo Geraldo
    Fernandes, Christian
    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2020, 116
  • [9] Solid-Phase Synthesis of Molecularly Imprinted Polymer Nanoparticles with a Reusable Template-"Plastic Antibodies"
    Poma, Alessandro
    Guerreiro, Antonio
    Whitcombe, Michael J.
    Piletska, Elena V.
    Turner, Anthony P. F.
    Piletsky, Sergey A.
    ADVANCED FUNCTIONAL MATERIALS, 2013, 23 (22) : 2821 - 2827
  • [10] Use of polymeric solid phase in synthesis of MIP nanoparticles for biotin
    Abbott, Benjamin
    Bedwell, Thomas S.
    Grillo, Fabiana
    Piletsky, Stanislav
    Whitcombe, Michael J.
    Piletska, Elena
    Garcia-Cruz, Alvaro
    Cowen, Todd
    Piletsky, Sergey A.
    REACTIVE & FUNCTIONAL POLYMERS, 2022, 170
12345