Sensitive ELISA test for determination of ochratoxin A

被引：72
|
作者：
Barna-Vetro, I
Solti, L
Teren, J
Gyongyosi, A
Szabo, E
Wolfling, A
机构：
[1] UNIV VET SCI BUDAPEST, H-1400 BUDAPEST, HUNGARY
[2] ANIM HLTH & FOOD CONTROL STN, H-6701 SZEGED, HUNGARY
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1996年 / 44卷 / 12期
关键词：
ochratoxin A; ELISA; cereals;
D O I：
10.1021/jf960442n
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
A direct, competitive enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody has been developed for quantitative determination of ochratoxin A (OA) in different cereals. A dichloromethane/citric acid mixture was used for extraction of cereals. This cleanup procedure proved to be as effective for ochratoxin A extraction as protocols using strong acids. The mean within-assay and interassay coefficients of variation for the standard curve was <10%. The range of this test is 1-10 ng/g, with a detection limit of 0.5 ng/g OA. The toxin recovery from cereals infected with 5-100 ng/mL OA varied between 90 and 130%.
引用
收藏
页码：4071 / 4074
页数：4
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