Hematoxylin-eosin-methylene Blue Staining in a Dog Thromboembolism Case

A post-mortem examination of a 5 years old Rottweiler dog was performed in order to find out the death cause. Macroscopically examination of lungs highlighted on the pulmonary trunk thrombi which were spread from the origin of the trunk to each pulmonary artery. The dissection of the heart has revealed vegetative structure at the level of the valvular endocardium. For histopathological examination samples were taken from a thrombus and from the left valvular endocardium. For microscopic examination the samples were stained by chemical method using hematoxylin-eozine-methylene blue. Based on the chemical staining the histopathological changes and morphopathological aspects the diagnosis of pulmonary thromboembolism has been established.