Antioxidant interaction of α-tocopherol, γ-oryzanol and phytosterol in rice bran oil

In this study, purified rice bran oil (RBO) was used as a lipid matrix model to study the individual and binary antioxidant capacity of the minor constituents (alpha-tocopherol, gamma-oryzanol and phytosterol) added at different concentrations and ratios. The results revealed that concentration influenced on the oxidation stability and scavenging capacity, while ratio mainly affected the type of interaction or the degree of synergism or antagonism. It was important to notice that the antioxidant capacity of alpha-tocopherol would decrease under high concentration. Besides, the inhibition of phytosterol on alpha-tocopherol and the formation of hydrogen bond between gamma-oryzanol and phytosterol were speculated by the interactions of these minor constituents. This work helps to select efficient combinations for stabilizing the anti-oxidation of nutrient enriched RBO or provide suggestions for moderate retain of minor constituents in RBO.