Strand ligation in a double-stranded DNA by T4 RNA ligase

被引：2
|
作者：
Kinoshita, Y
Nishigaki, K
Husimi, Y
机构：
[1] Dept. of Funct. Materials Science, Saitama University, Urawa 338
来源：
CHEMISTRY LETTERS | 1996年 / 09期
关键词：
D O I：
10.1246/cl.1996.797
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
T4 RNA ligase was shown to ligate two DNA strands contained in a double helix with a high efficiency. Experiments on substrate and temperature dependence of this reaction supported that single-stranded ends clamped by a double-stranded region are necessary for this reaction. This finding is widely useful for DNA joining.
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页码：797 / 798
页数：2
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