Sequencing double-stranded DNA by strand displacement

被引：5

作者：

Fu, DJ

Koster, H

Smith, CL

Cantor, CR

机构：

[1] SEQUENOM INC,SAN DIEGO,CA 92121

[2] BOSTON UNIV,CTR ADV BIOTECHNOL,BOSTON,MA 02215

[3] BOSTON UNIV,DEPT BIOMED ENGN,BOSTON,MA 02215

[4] BOSTON UNIV,DEPT PHARMACOL,BOSTON,MA 02215

[5] BOSTON UNIV,DEPT EXPT THERAPEUT,BOSTON,MA 02215

[6] BOSTON UNIV,DEPT BIOL,BOSTON,MA 02215

[7] UNIV HAMBURG,DEPT BIOCHEM & MOL BIOL,D-20146 HAMBURG,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 03期

关键词：

D O I：

10.1093/nar/25.3.677

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Duplex probes with five base single-stranded overhangs can capture dsDNA targets from type IIS restriction nuclease digests. Ligation generates a predesigned nick site, where DNA polymerase can generate sequencing ladders by strand displacement or nick translation in the presence of trace amounts of dideoxynucleotides. This allows dsDNA targets to be captured from mixtures and directly sequenced without subcloning, purification or denaturation.

引用

页码：677 / 679

页数：3

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