Permanent cover crops in vineyard inter-row with white clover, alfalfa and tall fescue, clean tillage as the control. The effect of cover croping on soil microbial communities and seasonal variation were studied. The results showed that soil microorganism quantity decreased with deeper of soil layer, the differences were significant or very significant, and they were all higher in 0 similar to 20 cm, bacteria was 90% of total microorganism amount, followed by actinomyces and azotobacter. soil microorganism quantity take on some regularity with seasonal change, thereinto, the quantity of bacteria, actinomyces, azotobacter were apparently larger in July and September than that in April and December, especialy largest in July. The number of fungi was higher in April and December and lower in July and September. With comparison to clean tillage, the number of soil bacteria, fungi, azotobacter increased under cover crop treatments, there were significant differences between cover crop treatment of white clover, alfalfa and the control.