KINETICS OF GROWTH AND AMYLASE BIOSYNTHESIS OF SACCHAROMYCOPSIS FIBULIGERA DURING CULTIVATION IN BIOREACTOR

被引:0
|
作者
Nowak, Dorota [1 ]
Nowak, Agnieszka [1 ]
机构
[1] Szkola Glowna Gospodarstwa Wiejskiego, Katedra Inzynierii Zywnosci & Organizacji Prod, Wydz Nauk Zywnosci, PL-02776 Warsaw, Poland
来源
ZYWNOSC-NAUKA TECHNOLOGIA JAKOSC | 2009年 / 16卷 / 01期
关键词
potato starch; tapioca starch; modified starch; inductive medium; production of amylases; STARCH;
D O I
暂无
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The objective of this paper was to estimate the effect caused by the application of various sources of carbon in culture medium on the biosynthesis of amylase enzymes during cultivation of Saccharomycopsis fibuligera yeast in a bioreactor. In the inductive media, starch content constituted 2% and consisted of native potato starch, modified potato starch, or native tapioca starch. During cultivation, optical density (OD), biomass yield, and amylase activity were measured in the samples collected. The Bernfeld method was used to determine the amylase activity. The highest biomass gain (14 g(d.m.)/dm(3)) and the highest amylase activity (35 mu mol maltose/(cm(3) min) in the culture medium were obtained when the native potato starch was a source of carbon in the culture medium.
引用
收藏
页码:28 / 36
页数:9
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