A cell-free approach to accelerate the study of protein-protein interactions in vitro

被引：33
|
作者：
Sierecki, E. [1 ]
Giles, N. [1 ]
Polinkovsky, M. [1 ]
Moustaqil, M. [1 ]
Alexandrov, K. [1 ]
Gambin, Y. [1 ]
机构：
[1] Univ Queensland, Inst Mol Biosci, St Lucia, Qld, Australia
来源：
INTERFACE FOCUS | 2013年 / 3卷 / 05期
基金：
英国医学研究理事会; 澳大利亚国家健康与医学研究理事会;
关键词：
protein-protein interactions; drug discovery; in vitro protein expression; AlphaScreen; single-molecule fluorescence; SMALL-MOLECULE INHIBITORS; P53-MDM2; INTERACTION; P53; DISCOVERY; ASSAY; PATHWAY; COMPLEX; BINDING; SYSTEM; FUTURE;
D O I：
10.1098/rsfs.2013.0018
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Protein-protein interactions are highly desirable targets in drug discovery, yet only a fraction of drugs act as binding inhibitors. Here, we review the different technologies used to find and validate protein-protein interactions. We then discuss how the novel combination of cell-free protein expression, AlphaScreen and single-molecule fluorescence spectroscopy can be used to rapidly map protein interaction networks, determine the architecture of protein complexes, and find new targets for drug discovery.
引用
收藏
页数：10
相关论文
共 50 条
  • [11] Free energy decomposition of protein-protein interactions
    Noskov, SY
    Lim, C
    BIOPHYSICAL JOURNAL, 2001, 81 (02) : 737 - 750
  • [12] Cell-free Protein Synthesis in an Autoinduction System for NMR Studies of Protein–Protein Interactions
    Kiyoshi Ozawa
    Slobodan Jergic
    Jeffrey A. Crowther
    Phillip R. Thompson
    Gene Wijffels
    Gottfried Otting
    Nicholas A. Dixon
    Journal of Biomolecular NMR, 2005, 32 : 235 - 241
  • [13] New approach for predicting protein-protein interactions
    Bulletin of the Chinese Academy of Sciences, 2007, (02) : 76 - 76
  • [14] A hybrid approach to extract protein-protein interactions
    Bui, Quoc-Chinh
    Katrenko, Sophia
    Sloot, Peter M. A.
    BIOINFORMATICS, 2011, 27 (02) : 259 - 265
  • [15] How to Study Protein-protein Interactions
    Podobnik, Marjetka
    Krasevec, Nada
    Zavec, Apolonija Bedina
    Naneh, Omar
    Flasker, Ajda
    Caserman, Simon
    Hodnik, Vesna
    Anderluh, Gregor
    ACTA CHIMICA SLOVENICA, 2016, 63 (03) : 424 - 439
  • [16] A Simple Approach for Predicting Protein-Protein Interactions
    Rashid, Mamoon
    Ramasamy, Sumathy
    Raghava, Gajendra P. S.
    CURRENT PROTEIN & PEPTIDE SCIENCE, 2010, 11 (07) : 589 - 600
  • [17] KINETIC STUDY OF PROTEIN-PROTEIN INTERACTIONS
    KOREN, R
    HAMMES, GG
    BIOCHEMISTRY, 1976, 15 (05) : 1165 - 1170
  • [18] The study of protein-protein interactions in bacteria
    Velasco-Garcia, Roberto
    Vargas-Martinez, Roco
    CANADIAN JOURNAL OF MICROBIOLOGY, 2012, 58 (11) : 1241 - 1257
  • [19] Methods of study of protein-protein interactions
    Wildova, Marcela
    Rumlova, Michaela
    CHEMICKE LISTY, 2008, 102 (01): : 28 - 34
  • [20] In vitro synthetic biology: Cell-free protein synthesis
    Liu Y.
    Guo X.
    Geng J.
    Jiao Y.
    Han J.
    Zhang Z.
    Zhou X.
    Yang D.
    Yang, Dayong (dayong.yang@tju.edu.cn), 2017, Chinese Academy of Sciences (62): : 3851 - 3860
12345