The Guizhou-Yunnan provincial border is the epidemic area of HIV/AIDS, and the HIV infection rate is high among ethnic minority migrants. This cross-sectional survey was conducted on the high risk sexual behavior for HIV/AIDS from July to October in 2012 in the factories, construction sites, and service establishments in Xingyi city, Qixingguan district, and Weining county, Guizhou province. 845 migrants with the experience of sexual intercourse were interviewed. The results show that the probability of the high risk sexual behavior for HIV/AIDS was higher among ethnic minority migrants (especially among ethnic Miao and Bouyi) than among ethnic Han migrants, and influenced by migration type, and number of floating city. The results suggest that the enterprise and government agency should improve the working condition and benefit of ethnic minority migrants to reduce the high risk sexual behavior for HIV/AIDS.