A new method of recovering polyhydroxyalkanoate from Azotobacter chroococcum

被引：51
|
作者：
Dong, ZL [1 ]
Sun, XN [1 ]
机构：
[1] NW Univ Xian, Dept Biol, Key Lab Biotechnol Shannxi, Xian 710069, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2000年 / 45卷 / 03期
关键词：
freezing treatment; separation; purification; polyhydroxyalkanoate (PHA);
D O I：
10.1007/BF02884685
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
After polyhydroxyalkanoate (PHA) fermentation for 42-48 h by the Azotobacter chroococcum G-3, the PHA content reached more than 75% of the dry weight. Biomass was isolated from culture by centrifugation and pretreated with freezing to release PHA pellet, then was treated with 10 g/L sodium dodecyl sulfate (SDS) for 15 min to effectively solubilize lipid and protein. Subsequently, it was further purified by digesting with 30% sodium hypochlorite (NaClO) for 3 min to remove peptidoglycan and non-PHA biomass. Finally, 98% PHA was obtained by diluting and rinsing with water, and the PHA recovered was suitable for processing.
引用
收藏
页码：252 / 256
页数：5
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