A cationic derivative of amphotericin B as a novel delivery system for antisense oligonucleotides

被引：13
|
作者：
Garcia-Chaumont, C [1 ]
Seksek, O [1 ]
Jolles, B [1 ]
Bolard, J [1 ]
机构：
[1] Univ Paris 06, Lab Physicochim Biomol & Cellulaire, CNRS, ESA 7033, F-75252 Paris 05, France
来源：
ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT | 2000年 / 10卷 / 03期
关键词：
D O I：
10.1089/oli.1.2000.10.177
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel approach based on a plasma membrane permeability-disturbing agent was proposed as an antisense oligonucleotide delivery system. AMA, a derivative of the polyene antibiotic amphotericin B, formed a stable complex when mixed with phosphodiester oligodeoxynucleotides and enhanced the intracellular uptake of a 5' fluoresceinated anti-mdr1 20-mer into NIH-MDR-G185 cells. The nonlabeled phosphorothioate form of the oligodeoxynucleotide, complexed to AMA, inhibited P-glycoprotein expression with better efficiency and less nonspecific effects than when vectorized by Lipofectin. AMA may thus be a good agent for antisense strategy.
引用
收藏
页码：177 / 184
页数：8
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