Application of an indirect ELISA and a PCR technique for detection of cows' milk in sheep's and goats' milk cheeses

被引：53

作者：

Lopez-Calleja, Ines M. ^{[1
]}

Gonzalez, Isabel ^{[1
]}

Fajardo, Violeta ^{[1
]}

Hernandez, Pablo E. ^{[1
]}

Garcia, Teresa ^{[1
]}

Martin, Rosario ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

来源：

INTERNATIONAL DAIRY JOURNAL | 2007年 / 17卷 / 01期

关键词：

PCR; indirect ELISA; cow; sheep; goat; cheese; 12S rRNA gene;

D O I：

10.1016/j.idairyj.2006.01.006

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

We have applied a polymerase chain reaction (PCR) procedure and an indirect enzyme-linked immunosorbent assay (ELISA) for detecting the presence of cows' milk in sheeps' and goats' milk cheeses. The PCR used a cattle-specific primer set targeting a 223 bp fragment from the mitochondrial 12S rRNA gene. This technique was applied to experimental cheese mixtures, industrial cheeses produced with known amounts of cow's milk, and several commercial cheeses, enabling the detection of low percentages of cows' milk (1%). An indirect ELISA using a monoclonal antibody (AH4) against bovine beta-casein was also assayed in this study for the specific detection of bovine milk in cheese. Results suggested that both ELISA and PCR may provide specific and reliable tools for detection of low percentages of undeclared cows' milk in sheeps' and/or goats' milk cheeses and other dairy products. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：87 / 93

页数：7

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