Heme Oxygenase-2 Suppress TNF-α and IL6 Expression via TLR4/MyD88-Dependent Signaling Pathway in Mouse Cerebral Vascular Endothelial Cells

被引：19
|
作者：
Chen, Ren-Jin [1 ]
Yuan, Hong-Hua [2 ]
Zhang, Teng-Ye [2 ]
Wang, Zhen-Zhen [2 ]
Hu, An-kang [1 ]
Wu, Lian-Lian [2 ]
Yang, Zhang-Ping [3 ]
Mao, Yong-Jiang [3 ]
Ji, De-Jun [3 ]
Zhu, Xiao-Rong [1 ]
机构：
[1] Xuzhou Med Coll, Lab Anim Ctr, Xuzhou 221004, Jiangsu, Peoples R China
[2] Xuzhou Med Coll, Neurobiol Res Ctr, Xuzhou, Jiangsu, Peoples R China
[3] Yangzhou Univ, Anim Sci & Technol Coll, Yangzhou, Jiangsu, Peoples R China
来源：
MOLECULAR NEUROBIOLOGY | 2014年 / 50卷 / 03期
关键词：
Heme oxygenase-2; Tumor necrosis factor alpha; Interleukin-6; CLI-095; Cerebral vascular endothelial cells; TOLL-LIKE RECEPTORS; ALZHEIMERS-DISEASE; OXIDATIVE STRESS; APOPTOSIS; HO-2; INJURY; INFLAMMATION; RECOGNITION; GLUTAMATE; TAK-242;
D O I：
10.1007/s12035-014-8693-x
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Heme oxygenase (HO) represents an intrinsic antiinflammatory system based on its ability to inhibit expression of proinflammatory cytokines. The constitutive isoform heme oxygenase-2 (HO-2) has high expression and activity in cerebral microvascular endothelial cells (CMVEC). This study was undertaken to evaluate the role of HO-2 in regulation of TLR4/MyD88-dependent signaling and to study the effect of HO-2 on the expression and secretion of the proinflammatory cytokines tumor necrosis factor alpha (TNF-alpha) and Interleukin-6 (IL6) in CMVEC. HO-2 short hairpin RNA (shRNA) and HO-2 overexpression plasmids were used to observe the effect of HO-2 on proinflammatory cytokines in CMVEC in vitro, and the results showed that the messenger RNA (mRNA) and protein levels of TNF-alpha and IL6 were increased and decreased, respectively, compared with control groups. LPS-stimulated TNF-alpha and IL6 mRNA and protein were also reduced in CMVEC treated with an inhibitor of TLR4 signaling, CLI-095, or HO-2 overexpression. CLI-095 and HO-2 overexpression both reduced TLR4 expression in CMVEC, and HO-2 shRNA blocked these effects of CLI-095. CLI-095 and HO-2 overexpression potently suppressed TLR4/MyD88-dependent proinflammatory cytokine expression in CMVEC. These results suggest that HO-2 plays an important role in protecting CMVEC against cytokine-mediated inflammation.
引用
收藏
页码：971 / 978
页数：8
相关论文
共 46 条
  • [21] TLR4 regulates lung injury and neutrophil activation via TRIF, and to a lesser extent, MyD88-dependent signaling pathways in a mouse trauma-hemorrhagic shock lymph infusion model
    Reino, Diego
    Feinman, Rena
    Xu, Da-Zhong
    Feketeova, Eleonora
    Palange, David
    Deitch, Edwin
    INFLAMMATION RESEARCH, 2010, 59 : S114 - S115
  • [22] Stearic Acid and TNF-α Co-Operatively Potentiate MIP-1α Production in Monocytic Cells via MyD88 Independent TLR4/TBK/IRF3 Signaling Pathway
    Kochumon, Shihab
    Arefanian, Hossein
    Azim, Rafaat
    Shenouda, Steve
    Jacob, Texy
    Abu Khalaf, Nermeen
    Al-Rashed, Fatema
    Hasan, Amal
    Sindhu, Sardar
    Al-Mulla, Fahd
    Ahmad, Rasheed
    BIOMEDICINES, 2020, 8 (10) : 1 - 16
  • [23] Early continuous blood purification affects TNF-α, IL-1β, and IL-6 in patients with severe acute pancreatitis via inhibiting TLR4 signaling pathway
    Chen, Zhi-Peng
    Huang, Hai-Ping
    He, Xiao-Yan
    Wu, Bao-Zhen
    Liu, Yong
    KAOHSIUNG JOURNAL OF MEDICAL SCIENCES, 2022, 38 (05): : 479 - 485
  • [24] TLR-1, TLR-2, and TLR-6 MYD88-dependent signaling pathway: A potential factor in the interaction of high-DNA fragmentation human sperm with fallopian tube epithelial cells
    Zandieh, Zahra
    Govahi, Azam
    Aghamajidi, Azin
    Raoufi, Ehsan
    Amjadi, Fatemehsadat
    Aghajanpour, Samaneh
    Golestan, Masoomeh
    Aflatoonian, Reza
    CLINICAL AND EXPERIMENTAL REPRODUCTIVE MEDICINE-CERM, 2023, 50 (01): : 44 - 52
  • [25] Jellyfish collagen stimulates production of TNF-α and IL-6 by J774.1 cells through activation of NF-κB and JNK via TLR4 signaling pathway
    Putra, Agus Budiawan Naro
    Nishi, Kosuke
    Shiraishi, Ryusuke
    Doi, Mikiharu
    Sugahara, Takuya
    MOLECULAR IMMUNOLOGY, 2014, 58 (01) : 32 - 37
  • [26] The extract of Ramulus Cinnamom attenuates inflammatory injury induced by LPS via regulation of TLR4/MyD88 signaling pathway in BV2 cells
    YANG Huan
    CHENG Xiao
    ZHANG Jun
    YANG Yin-lin
    WANG Yue-hua
    DU Guan-hua
    中国药理学与毒理学杂志, 2016, (10) : 1029 - 1030
  • [27] Swimming exercise improves gene expression of PPAR-γ and downregulates the overexpression of TLR4, MyD88, IL-6, and TNF-α after high-fat diet in rat skeletal muscle cells
    Shirvani, Hossein
    Mirnejad, Reza
    Soleimani, Mehdi
    Arabzadeh, Ehsan
    GENE, 2021, 775
  • [28] Integrin β3 positively regulates Toll-like receptor (TLR)-triggered inflammatory responses by targeting CD14 expression via a TLR4/MyD88-dependent and TRIF-independent pathway in murine sepsis
    Chen, Z.
    Ding, X.
    Mei, S.
    Jin, S.
    Tong, Y.
    Li, Q.
    BRITISH JOURNAL OF ANAESTHESIA, 2016, 116 (06) : E917 - E918
  • [29] A malaria protein factor induces IL-4 expression by dendritic cells via TLR/MyD88-independent signaling, promoting Th2 development
    Wu, Xianzhu
    Gowda, Nagaraj M.
    Gowda, Channe D.
    JOURNAL OF IMMUNOLOGY, 2018, 200 (01):
  • [30] Lipopolysaccharide induces ICAM-1 expression in human pulmonary alveolar epithelial cells through a TLR4/MyD88/TRAF6/NADPH oxidase-dependent pathway
    Cho, Rou-Ling
    Yang, Chuen-Mao
    FASEB JOURNAL, 2012, 26
12345