Exercise Training Impacts Skeletal Muscle Clock Machinery in Prediabetes

被引:19
|
作者
Erickson, Melissa L. [1 ]
Zhang, Hui [1 ,2 ]
Mey, Jacob T. [1 ]
Kirwan, John P. [1 ,2 ]
机构
[1] Louisiana State Univ, Pennington Biomed Res Ctr, Integrat Physiol & Mol Med Lab, Baton Rouge, LA 70803 USA
[2] Case Western Univ, Dept Physiol & Biophys, Cleveland, OH USA
基金
美国国家卫生研究院;
关键词
EXERCISE; SKELETAL MUSCLE MOLECULAR CLOCK; INSULIN SENSITIVITY; CIRCADIAN RHYTHMS; OBESITY; CIRCADIAN CLOCK; INSULIN SENSITIVITY; GENE-EXPRESSION; METABOLISM; TIME; OBESITY;
D O I
10.1249/MSS.0000000000002368
中图分类号
G8 [体育];
学科分类号
04 ; 0403 ;
摘要
Purpose Disruption of the skeletal muscle molecular clock leads to metabolic disease, whereas exercise may be restorative, leading to improvements in metabolic health. The purpose of this study was to evaluate the effects of a 12-wk exercise intervention on skeletal muscle molecular clock machinery in adults with obesity and prediabetes, and determine whether these changes were related to exercise-induced improvements in metabolic health. Methods Twenty-six adults (age, 66 +/- 4.5 yr; body mass index (BMI), 34 +/- 3.4 kg center dot m(-2); fasting plasma glucose, 105 +/- 15 mg center dot dL(-1)) participated in a 12-wk exercise intervention and were fully provided isoenergetic diets. Body composition (dual x-ray absorptiometry), abdominal adiposity (computed tomography scans), peripheral insulin sensitivity (euglycemic-hyperinsulinemic clamp), exercise capacity (maximal oxygen consumption), and skeletal muscle molecular clock machinery (vastus lateralisbiopsy) were assessed at baseline and after intervention. Gene and protein expression of skeletal muscle BMAL1, CLOCK, CRY1/2, and PER 1/2 were measured by quantitative real-time polymerase chain reaction and Western blot, respectively. Results Body composition (BMI, dual x-ray absorptiometry, computed tomography), peripheral insulin sensitivity (glucose disposal rate), and exercise capacity (maximal oxygen consumption) all improved (P< 0.005) with exercise training. Skeletal muscleBMAL1gene (fold change, 1.62 +/- 1.01;P= 0.027) and PER2 protein expression (fold change, 1.35 +/- 0.05;P= 0.02) increased, whereas CLOCK, CRY1/2, and PER1 were unchanged. The fold change inBMAL1correlated with post-glucose disposal rate (r= 0.43,P= 0.044), BMI (r= -0.44,P= 0.042), and body weight changes (r= -0.44,P= 0.039) expressed as percent delta. Conclusions Exercise training impacts skeletal muscle molecular clock machinery in a clinically relevant cohort of adults with obesity and prediabetes. Skeletal muscleBMAL1gene expression may improve insulin sensitivity. Future studies are needed to determine the physiological significance of exercise-induced alterations in skeletal muscle clock machinery.
引用
收藏
页码:2078 / 2085
页数:8
相关论文
共 50 条
  • [41] Exercise training increases skeletal muscle LDL uptake, in mice
    Vinagre, CGC
    Finazzo, C
    Angelis, K
    Irigoyen, M
    Maranhao, R
    ATHEROSCLEROSIS SUPPLEMENTS, 2004, 5 (01) : 156 - 156
  • [42] Effect of exercise training on skeletal muscle cytokine expression in the elderly
    Della Gatta, Paul A.
    Garnham, Andrew P.
    Peake, Jonathan M.
    Cameron-Smith, David
    BRAIN BEHAVIOR AND IMMUNITY, 2014, 39 : 80 - 86
  • [43] Exercise Training Enhances Mitochondrial Fusion in Human Skeletal Muscle
    Axelrod, Christopher L.
    Mulya, Anny
    Fealy, Ciaran E.
    Hoppel, Charles L.
    Kirwan, John P.
    DIABETES, 2017, 66 : A16 - A16
  • [44] Skeletal muscle biochemical adaptations to exercise training in miniature swine
    McAllister, RM
    Reiter, BL
    Amann, JF
    Laughlin, MH
    JOURNAL OF APPLIED PHYSIOLOGY, 1997, 82 (06) : 1862 - 1868
  • [45] Exercise and training effects on ceramide metabolism in human skeletal muscle
    Helge, JW
    Dobrzyn, A
    Saltin, B
    Gorski, J
    EXPERIMENTAL PHYSIOLOGY, 2004, 89 (01) : 119 - 127
  • [46] Effect of Aging and Exercise Training on Mitochondrial Function in Skeletal Muscle
    Ghosh, Sangeeta A.
    Lertwattanarak, Raweewan
    Cervera-Cetina, Antonio
    Molina, Marjorie
    Joya, Joaquin
    Abdul-Ghani, Muhammad
    Richardson, Arlan G.
    Van Remmen, Holly
    Defronzo, Ralph A.
    Musi, Nicolas
    DIABETES, 2009, 58 : A66 - A67
  • [47] Adaptive responses in skeletal muscle of mice following exercise training
    Kayani, AC
    Broome, CS
    Jackson, MJ
    McArdle, A
    FASEB JOURNAL, 2004, 18 (05): : A1092 - A1092
  • [48] Regulation of skeletal muscle hexokinase (HK) by exercise training.
    Halseth, AE
    Bracy, DB
    Namdaran, K
    Rowe, S
    Wasserman, DH
    FASEB JOURNAL, 1997, 11 (03): : 1682 - 1682
  • [49] Expression of phospholemman in aged skeletal muscle: Effect of exercise training
    Reis, JJ
    Cala, SE
    Jew, KN
    Mace, LC
    Moore, RL
    Ng, YC
    FASEB JOURNAL, 2004, 18 (04): : A346 - A346
  • [50] Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle
    Benziane, Boubacar
    Widegren, Ulrika
    Pirkmajer, Sergej
    Henriksson, Jan
    Stepto, Nigel K.
    Chibalin, Alexander V.
    AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2011, 301 (03): : E456 - E466