Characterization of Lin-ve CD34 and CD117 Cell Population Reveals an Increased Expression in Bone Marrow Derived Stem Cells

被引：12

作者：

Jindal, Neeru ^{[1
]}

Minhas, Gillipsie ^{[1
]}

Prabhakar, Sudesh ^{[2
]}

Anand, Akshay ^{[1
]}

机构：

[1] PGIMER, Dept Neurol, Neurosci Res Lab, Chandigarh 160012, India

[2] PGIMER, Dept Neurol, Chandigarh 160012, India

来源：

CURRENT NEUROVASCULAR RESEARCH | 2014年 / 11卷 / 01期

关键词：

Bone marrow; CD34; CD45; CD117; EPCs; Sca1; Stem cell; C-KIT; FLOW-CYTOMETRY; MOUSE; SUBPOPULATIONS; PURIFICATION; BRAIN; VIVO;

D O I：

10.2174/1567202610666131209110035

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

The purpose of the study was to evaluate the expression of CD45, CD34, Sca-1 and CD117 in mouse bone marrow, Lin-ve and Lin+ve population. Bone marrow cells were isolated from C57/BL6J mouse and mononuclear population was separated from rest of the cell population. With the help of Magnetic associated cell sorter ( MACS), Lin-ve and Lin+ve cells were separated from the bone marrow. The expression of CD45, CD34, Sca1 and CD117 was evaluated in bone marrow, Lin-ve and Lin+ve population by flow cytometry. We found a significant increase in the expression of CD34 and CD117 in Lin-ve as compared to the bone marrow and Lin+ve population. These findings suggest that Lin-ve population has higher expression of stem cell progenitor markers and could be useful for tissue repair and regeneration.

引用

页码：68 / 74

页数：7

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