Development of a three-dimensional organ culture model for corneal wound healing and corneal transplantation

被引:31
|
作者
Zhao, Bojun
Cooper, Leanne J.
Brahma, Arun
MacNeil, Sheila
Rimmer, Stephen
Fullwood, Nigel J. [1 ]
机构
[1] Univ Lancaster, Dept Sci Biol, Lancaster LA1 4YQ, England
[2] Manchester Royal Eye Hosp, Manchester, Lancs, England
[3] No Gen Hosp, Div Clin Sci, Sheffield S5 7AU, S Yorkshire, England
[4] Kroto Res Inst, Dept Mat Engn, Sheffield, S Yorkshire, England
[5] Univ Sheffield, Dept Chem, Polymer & Biomat Chem Labs, Sheffield, S Yorkshire, England
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1167/iovs.05-1367
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
PURPOSE. To develop and evaluate a three-dimensional organ culture system of the cornea anterior chamber that could replicate the in vivo processes occurring during corneal wound healing and corneal transplantation. METHODS. Bovine corneoscleral buttons were clamped in a specially designed chamber through the sclera outside the limbus. The epithelium was exposed to air, and its anterior surface was automatically irrigated. The endothelial layer was perfused separately with media under normal intraocular pressure. Wound healing and corneal transplantation were observed using light, scanning, and transmission electron microscopy. RESULTS. The organ culture system maintained the epithelium, the putative epithelial stem cells in the limbus, the stroma, and the endothelium in good condition for the 10-day period during which the system was evaluated. The authors observed that the processes of wound healing and corneal transplantation in the model appeared similar to those occurring in vivo. CONCLUSIONS. In vitro model closely replicated the in vivo processes of wound healing and corneal transplantation. The authors believe this model will be useful for basic investigations into the cornea, such as study of the response of the cornea to surgery, wound healing, toxins, and therapeutic agents.
引用
收藏
页码:2840 / 2846
页数:7
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