Protective and Antioxidant Role of Selenium Nanoparticles and Vitamin C Against Acrylamide Induced Hepatotoxicity in Male Mice

Background and Objectives: Selenium (SeNPs) and vitamin C have a lot of significant protective antioxidant effects. Acrylamide (ACR) is found in starchy foods and breads that has been heated over long periods over 120 degrees C. The current study aimed to evaluate the ameliorative roles of SeNPs and/or vitamin C on acrylamide (ACR)-induced hepatotoxicity in male mice. Materials and Methods: Total 42 male mice were divided randomly to seven groups: Control, ACR, SeNPs, vitamin C,ACR plus SeNPs,ACR plus vitamin C,ACR plus SeNPs and vitamin C. Hepatic enzymes levels (Alanine and aspartate aminotransferases) and tumor necrosis factor-alpha as well as the antioxidant markers: Superoxide dismutase, glutathione-S-transferase, catalase and glutathione red uctase were evaluated. Histological, transmission electron microscopic and comet assay alterations in the hepatic cells as markers of hepatic damage after the ACR administration alone or combined with SeNPs and/or vitamin C were done. The ACR genotoxic effects were also assessed and the ameliorative roles of SeNPs and/or vitamin Con the liver cells were recorded as well. Results: Results clarified that vitamin C and SeNPs administration declined ACR-elicited increment in aminotransferase levels and declined oxidative stress significantly. Additionally, vitamin C combined with SeNPs had a potent synergistic effects and increased the antioxidant enzymes significantly and declined lipid peroxidation markers as compared with ACR-group. Conclusion: It is apparent that both vitamin C and SeNPs prohibit the liver damage and enhance the redox state in male mice.