Chitosan isolation from the chitosan-glucan complex of fungal cell wall using amylolytic enzymes

被引：13
|
作者：
Nwe, N [1 ]
Stevens, WF [1 ]
机构：
[1] Asian Inst Technol, Sch Environm Resources & Dev, Proc Technol Program, Bangkok 12120, Thailand
来源：
BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 18期
关键词：
amylolytic enzyme; chitosan-glucan complex; fungal chitosan;
D O I：
10.1023/A:1019898715518
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The chitosan/glucan complex isolated from the mycelia of the fungus, Gongronella butleri USDB 0201 can be cleaved with a heat-stable alpha-amylase at 65 degreesC for 3 h. This results in the removal of the glucan side chain and gives a chitosan solution with 100 times lower turbidity. It is proposed that chitosan and glucan chains are bound by an alpha(1 to 4) glucosidic bond. Both fungal chitosan and fungal glucan have been purified separately.
引用
收藏
页码：1461 / 1464
页数：4
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