Alterations in Intestinal Microbiota of Children With Celiac Disease at the Time of Diagnosis and on a Gluten-free Diet

被引:48
|
作者
Zafeiropoulou, Konstantina [1 ]
Nichols, Ben [1 ]
Mackinder, Mary [1 ]
Biskou, Olga [1 ]
Rizou, Eleni [1 ]
Karanikolou, Antonia [1 ]
Clark, Clare [1 ]
Buchanan, Elaine [2 ]
Cardigan, Tracey [2 ]
Duncan, Hazel [2 ]
Wands, David [2 ]
Russell, Julie [3 ]
Hansen, Richard [2 ]
Russell, Richard K. [2 ]
McGrogan, Paraic [2 ]
Edwards, Christine A. [1 ]
Ijaz, Umer Z. [3 ]
Gerasimidis, Konstantinos [1 ]
机构
[1] Univ Glasgow, Glasgow Royal Infirm, Coll Med Vet & Life Sci, Sch Med Dent & Nursing,Human Nutr, New Lister Bldg, Glasgow G31 2ER, Lanark, Scotland
[2] Royal Hosp Children, Dept Paediat Gastroenterol, Glasgow, Lanark, Scotland
[3] Univ Glasgow, Sch Engn, Civil Engn, Glasgow, Lanark, Scotland
基金
英国生物技术与生命科学研究理事会;
关键词
OTU; Pediatric; Microbiome; Short-chain Fatty Acids;
D O I
10.1053/j.gastro.2020.08.007
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
BACKGROUND AND AIMS: It is not clear whether alterations in the intestinal microbiota of children with celiac disease (CD) cause the disease or are a result of disease and/or its treatment with a gluten-free diet (GFD). METHODS: We obtained 167 fecal samples from 141 children (20 with new onset CD, 45 treated with a GFD, 57 healthy children, and 19 unaffected siblings of children with CD) in Glasgow, Scotland. Samples were analyzed by 16S ribosomal RNA sequencing, and diet-related metabolites were measured by gas chromatography. We obtained fecal samples from 13 children with new-onset CD after 6 and 12 months on a GFD. Relationships between microbiota with diet composition, gastrointestinal function, and biomarkers of GFD compliance were explored. RESULTS: Microbiota a diversity did not differ among groups. Microbial dysbiosis was not observed in children with new-onset CD. In contrast, 2.8% (Bray-Curtis dissimilarity index, P = .025) and 2.5% (UniFrac distances, P = .027) of the variation in microbiota composition could be explained by the GFD. Between 3% and 5% of all taxa differed among all group comparisons. Eleven distinctive operational taxonomic units composed a microbe signature specific to CD with high diagnostic probability. Most operational taxonomic units that differed between patients on a GFD with new-onset CD vs healthy children were associated with nutrient and food group intake (from 75% to 94%) and with biomarkers of gluten ingestion. Fecal levels of butyrate and ammonia decreased during the GFD. CONCLUSIONS: Although several alterations in the intestinal microbiota of children with established CD appear to be effects of a GFD, specific bacteria were found to be distinct biomarkers of CD. Studies are needed to determine whether these bacteria contribute to pathogenesis of CD.
引用
收藏
页码:2039 / 2051.e20
页数:33
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