Mildly oxidised low density lipoprotein induces platelet shape change via Rho-kinase-dependent phosphorylation of myosin light chain and moesin

被引：39

作者：

Retzer, M ^{[1
]}

Siess, W ^{[1
]}

Essler, M ^{[1
]}

机构：

[1] Kreislaufkrankheiten, Inst Prophylaxe & Epidemiol, D-80336 Munich, Germany

来源：

FEBS LETTERS | 2000年 / 466卷 / 01期

关键词：

platelet shape change; Rho-GTPase; oxidized low density lipoprotein; Rho-kinase; myosin light chain phosphorylation; moesin phosphorylation;

D O I：

10.1016/S0014-5793(99)01762-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidised low density lipoprotein (LDL) plays an important role in the pathogenesis of atherosclerosis, Here me demonstrate that mildly oxidised (mox) LDL engages the GTPase Rho and its effector molecule p160 Rho-kinase to induce phosphorylation of myosin light chain and of moesin leading to platelet shape change, Pretreatment of platelets with the selective Rho inhibitor C3-transferase from Clostridium botulinum or with the Rho-kinase inhibitor Y-27632 blocked mox-LDL-induced myosin light chain phosphorylation, moesin phosphorylation and shape change. Mos-LDL did not induce an increase in cytosolic Ca2+ during shape change. We propose that Rho/Rho-kinase inhibition could be a strategy for prevention of the pathologic platelet activation during atherogenesis. (C) 2000 Federation of European Biochemical Societies.

引用

页码：70 / 74

页数：5