RETRACTED: Involvement of a Specific Chemosensory Protein from Bactrocera dorsalis in Perceiving Host Plant Volatiles (Retracted article. See vol. 42, pg. 461, 2016)

被引:23
|
作者
Yi, Xin [1 ]
Wang, PeiDan [1 ]
Wang, Zheng [1 ]
Cai, Jun [1 ]
Hu, MeiYing [1 ]
Zhong, GuoHua [2 ]
机构
[1] South China Agr Univ, Lab Insect Toxicol, Key Lab Pesticide & Chem Biol, Minist Educ, Guangzhou 510642, Guangdong, Peoples R China
[2] South China Agr Univ, Coll Nat Resources & Environm, Key Lab Pesticide & Chem Biol, Minist Educ,PR China, Guangzhou 510642, Guangdong, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
Bactrocera dorsalis; Chemosensory protein; Binding assay; RNA interference; Electroantennogram recording; ODORANT-BINDING PROTEINS; LOCUSTA-MIGRATORIA ORTHOPTERA; BACTROCERA-DORSALIS; PHEROMONE-BINDING; IMMUNOCYTOCHEMICAL LOCALIZATION; MOLECULAR CHARACTERIZATION; OLFACTORY SENSILLA; BOMBYX-MORI; EXPRESSION; INSECT;
D O I
10.1007/s10886-014-0406-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Insects have evolved many physiological and behavioral adaptations to recognize external complex chemicals. Olfaction plays an important role in perceiving volatile chemicals, utilizing them to locate host sites, conspecifics, and enemies. Chemosensory proteins (CSPs) are present in high concentrations within the sensory sensilla of insects and are endowed with a heterogeneous range of functions. However, direct evidence for the involvement of CSPs in olfactory function is still lacking. In this study, a fluorescence-based ligand binding assay using Bdor-CSP2 illustrated its ability to bind the majority of the selected ligands of different shapes and chemical structures that are ecologically significant, host plant volatiles of Bactrocera dorsalis. RNAi-mediated silencing coupled with electrophysiological tests showed lower electrophysiological responses to (3Z)-hex-3-en-1-ol, trans-2-hexenal, 6-methylhept-5-en-2one, and 3-methylbutyl acetate in dsBdor CSP2 treated flies compared with the untreated controls. The reduced expression of Bdor-CSP2 by RNA interference was confirmed by semiquantitative PCR, real-time quantitative PCR and Western blot, which suggested the RNAi-treatment was responsible for the observed reduction of antennal responses in EAG recordings. These data suggest that the expression of BdorCSP2 is necessary for the recognition of antennal responses to some plant host volatiles by B. dorsalis.
引用
收藏
页码:267 / 275
页数:9
相关论文
共 50 条