Amylibacter lutimaris sp nov., isolated from sea-tidal flat sediment

A Gram-stain-negative, aerobic bacterial strain, designated strain m18(T), was isolated from a sea-tidal flat in South Korea. Cells were non-motile short rods showing oxidase and catalase activities. Growth of m18(T)- was observed at 10-40 degrees C (optimum, 30 degrees C), pH 5.5-10.0 (optimum, pH 7.0) and 0.5-7.0 % (w/v) NaCl (optimum, 3.0 %). The major respiratory quinone was ubiquinone-10 and the major fatty acids of were summed feature 8 (comprising C-18:1 omega 7c/C-18:1 omega 6c) and C-16:0. The G+C content of the genomic DNA was 56.7 mol%. Phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified phospholipid, an unidentified aminolipid and four unidentified lipids were detected in m18(T). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that m18(T)- formed a tight phyletic lineage with the members of the genus Amylibacter. Strain m18(T)- was most closely related to Amylibactercionae H-12(T), Amylibacter ulvae 6Alg 255(T)- and Amylibacter marinus 2-3(T)- with 98.9, 96.1 and 95.5 % 16S rRNA gene sequence similarities, respectively. The DNA- DNA hybridization value between m18(T)- and the type strain of A. cionae was 43.6 +/- 3.4 %. On the basis of phenotypic, chemotaxonomic and molecular properties, m18(T)- represents a novel species of the genus Amylibacter, for which the name Amylibacter lutimaris sp. nov. is proposed. The type strain is m18(T)- (KACC 19229(T)=JCM 32051(T)).