Purification and characterization of a novel prolyl aminopeptidase from Maitake (Grifold frondosa)

被引：13

作者：

Hiwatashi, K

Hori, K

Takahashi, K

Kagaya, A

Inoue, S

Sugiyama, T

Takahashi, S

机构：

[1] Akita Res Inst Food & Brewing, Dept Bioengn, Akita 0101623, Japan

[2] Akita Jujo Chem Co Ltd, Akita 0101633, Japan

[3] Akita Univ, Sch Med, Dept Biochem, Akita 0108543, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2004年 / 68卷 / 06期

关键词：

Grifola frondosa; prolyl aminopeptidase; purification; characterization;

D O I：

10.1271/bbb.68.1395

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have found a novel prolyl aminopeptidase in Grifola frondosa. The enzyme was purified by DEAE-Sepharose CL-6B, Butyl-Toyopearl, Sephacryl S-100, and Mono-Q column chromatographies. The purified enzyme exists as a dimer and gives high activity toward L-proline-p-nitroanilide. The enzyme was strongly inhibited by p-chloromercuribenzoic acid and iodoacetic acid and markedly inhibited by phenylmethylsulfonyl fluoride and arphamenin A.

引用

页码：1395 / 1397

页数：3

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