Zymographic assay to differentiate Lactobacillus reuteri among other Lactobacillus

被引：3
|
作者：
Ledesma, P.
Gonzalez, C. F.
Breccia, J. D.
Sineriz, F.
机构：
[1] PROIMI Biotecnol, Planta Piloto Proc Ind Microbiol, RA-4000 San Miguel De Tucuman, Argentina
[2] Univ Nacl La Pampa, Fac Ciencias Exactas & Nat, Depto Quim, RA-6300 Santa Rosa, La Pampa, Argentina
来源：
SCIENCES DES ALIMENTS | 2006年 / 26卷 / 03期
关键词：
Lactobacillus reuteri; 1,3-propanediol : NAD oxidoreductase; zymogram; 1,3-propanediol;
D O I：
10.3166/sda.26.283-287
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A simple method is reported that allows the phenotypic differentiation of Lactobacillus reuteri strains among other Lactobacillus. The detection of the enzyme 1,3-propanediol:NAD oxidoreductase in non-denaturing PAGE, a constitutive enzyme of Lb. reuteri involved in the production of 1,3-propanediol, is used. Other member of the genus were reported as 1,3 propanediol producers (Lactobacillus brevis and Lactobacillus buchnen), both of their enzymes showed differential properties to that of Lb. reuteri. Under the conditions used in the study, the strain Lb. reuteri CRL1100 was found to produce 15 % more 1,3-PDL (11.5 +/- 0.41 gl(-1)) in comparison with the reference strain Lb. reuteri DSM 20016 (10.0 +/- 0.37 gl(-1)).
引用
收藏
页码：283 / 287
页数：5
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