Quantitation of Protopine in Fumaria parviflora L. Whole Plant Powder by High-Performance Liquid Chromatography

Present method describes a high performance liquid chromatographic determination of protopine from Fumaria parviflora Linn. The separation was carried out on Cosmosil (C-18) (250 mm x 4.6 mm, i.d.) 5 mu column. The mobile phase was buffer: methanol in the volume ratio of (50:50) (v/v). The detection and quantification was carried out using a UV-visible detector at 291 nm. Linear concentration range of protopine was observed to be 0.0533- 0.2133 mu g mL(-1). The developed method was validated to determine its accuracy, precision and stability by carrying out linearity and stability experiments. The method developed is simple, fast, accurate and precise and hence can applied for routine quality control analysis of Fumaria parviflora Linn. whole plant powder and quantitative determination of protopine.