Galectin-3 modulates carbohydrate-dependent thymocyte interactions with the thymic microenvironment

被引:0
|
作者
Villa-Verde, DMS
Silva-Monteiro, E
Jasiulionis, MG
Farias-de-Oliveira, SA
Brentani, RR
Savino, W
Chammas, R
机构
[1] Hop Necker Enfants Malad, CNRS, UMR 8603, F-75743 Paris, France
[2] Oswaldo Cruz Fdn, Inst Oswaldo Cruz, Dept Immunol, Lab Thymus Res, Rio De Janeiro, Brazil
[3] Ludwig Inst Canc Res, Sao Paulo Branch, Sao Paulo, Brazil
[4] USP, Fac Med, Expt Oncol Lab, BR-09500900 Sao Paulo, Brazil
关键词
thymus; galectin; microenvironmental cell; cell-to-cell interaction; adhesion molecule;
D O I
10.1002/1521-4141(200205)32:5<1434::AID-IMMU1434>3.0.CO;2-M
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The process of thymocyte differentiation occurs within the context of the thymic microenvironment, in which T cell precursors interact with thymic microenvironmental cells and extracellular matrix. Here we studied the expression of galectin-3, a beta-galactoside binding lectin, in the thymus of young adult mice. Galectin-3 was found mainly in the medulla and to a lesser extent in the cortex. We further showed that distinct microenvironmental elements, such as thymic epithelial cells, the epithelial component of thymic nurse complexes and phagocytic cells of the thymic reticulum produce, secrete and accumulate galectin-3 on the cell surface. Functionally, galectin-3-enriched medium inhibited in vitro thymocyte interactions with thymic microenvironmental cells, accelerated the release of thymocytes from thymic nurse cells and inhibited the reconstitution of these lymphoepithelial complexes. These effects were blocked by exogenous lactose (Galbeta1-4Glc), but not melibiose (Galalpha1-6Glc), and by a monospecific anti-galectin-3 antibody. Recombinant galectin-3 also inhibited thymocyte/thymic epithelial cell interactions. Our data indicate that intrathymically produced galectin-3 disrupts thymocyte/microenvironmental cell interactions, thus acting as a de-adhesion molecule.
引用
收藏
页码:1434 / 1444
页数:11
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