Regulation of hydrogen photoproduction in Rhodobacter sphaeroides batch culture by external oxidizers and reducers

被引：26
|
作者：
Gabrielyan, Lilit [1 ]
Sargsyan, Harutyun [2 ]
Hakobyan, Lilit [1 ]
Trchounian, Armen [2 ]
机构：
[1] Yerevan State Univ, Dept Biophys, Yerevan 0025, Armenia
[2] Yerevan State Univ, Dept Microbiol & Plants & Microbes Biotechnol, Fac Biol, Yerevan 0025, Armenia
来源：
APPLIED ENERGY | 2014年 / 131卷
关键词：
Hydrogen photoproduction; Redox potential; Redox reagents; F0F1-ATPase activity; Rhodobacter sphaeroides; FERMENTATIVE H-2 PRODUCTION; ESCHERICHIA-COLI; OXIDATION-REDUCTION; NITROGENASE; GROWTH; F0F1-ATPASE; MEMBRANE;
D O I：
10.1016/j.apenergy.2014.06.019
中图分类号：
TE [石油、天然气工业]; TK [能源与动力工程];
学科分类号：
0807 ; 0820 ;
摘要：
Photo-fermentative production of hydrogen (H-2) by purple bacterium Rhodobacter sphaeroides MDC6521 from Armenian mineral springs and its regulation by external reducers and oxidizers have been investigated. Reducers such as DL-dithiothreitol (DTT) and dithionite suppress bacterial growth but enhance H-2 yield of R. sphaeroides, whereas oxidizer ferricyanide inhibits both processes. The effect of DTT on DCCD-inhibited F0F1-ATPase activity of R. sphaeroides membrane vesicles has been analyzed too. DTT increases the DCCD-inhibited ATPase activity. Thus, more negative values of E-h by addition of DU might regulate F0F1-ATPase activity. The participation of the ATPase in redox sensing under photo-fermentative H-2 production is suggested. This enzyme might be a target, having a significant role in these processes of purple bacteria. (C) 2014 Elsevier Ltd. All rights reserved.
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页码：20 / 25
页数：6
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