New solid support for the synthesis of 3′-oligonucleotide conjugates through glyoxylic oxime bond formation

被引：13
|
作者：
Spinelli, Nicolas [1 ]
Edupuganti, Om Prakash [1 ]
Defrancq, Eric [1 ]
Dumy, Pascal [1 ]
机构：
[1] Univ Grenoble 1, Dept Chim Mol, UMR 5250, CNRS,ICMG FR2607, F-38041 Grenoble 9, France
来源：
ORGANIC LETTERS | 2007年 / 9卷 / 02期
关键词：
D O I：
10.1021/ol062607b
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A novel solid support 1 was synthesized to incorporate glyoxylic aldehyde functionality at the oligonucleotide 3'-terminus. 6-mer and 11-mer oligonucleotide sequences containing 3'-glyoxylic aldehyde functionality were prepared by using this support. These modified oligonucleotides were coupled to reporters containing an aminooxy group to prepare oligonucleotide 3'-conjugates through glyoxylic oxime bond formation. The hydrolytic stability of a glyoxylic oxime linkage was also investigated.
引用
收藏
页码：219 / 222
页数：4
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