Effect of polypeptide from Chlamys farreri on UVB-induced ROS/NF-κB/COX-2 activation and apoptosis in HaCaT cells

Polypeptide from Chlamys farreri (PCF) is a novel marine polypeptide compound isolated from gonochoric Chinese scallop Chlamys farreri, this study we further investigate the mechanisms of PCF exerting its antiapoptotic effect. The results indicated that PCF, ROS scavenger NAC and NF-kappa B inhibitor MG132 effectively inhibited UVB-induced HaCaT cells apoptosis. PCF (2.84 mM) showed potential ROS scavenging activities in a kinetic process. PCF (1.42-5.69 mM) dose-dependently increased the expressions of Cu, Zn-SOD, CAT and GPx: meanwhile decreased the expressions of p-NF-kappa B/p65 and COX-2 in UVB-induced HaCaT cells. Additionally, pretreatment with NAC significantly declined the generation of ROS and the expression of p-NF-kappa B/p65. We concluded that ROS, NF-kappa B and COX-2 are involved in UVB-induced HaCaT cells apoptosis, PCF exerts its protective effects via scavenging ROS, increasing the expression of antioxidative enzymes and inhibition the activation of NF-kappa B and COX-2. (C) 2009 Elsevier B.V. All rights reserved.