Rapid detection of t(15;17)(q24;q21) in acute promyelocytic leukaemia by microwave-assisted fluorescence in situ hybridization

被引：5
|
作者：
Soriani, Silvia [1 ]
Mura, Cinzia [1 ]
Panico, Anna Rita [1 ]
Scarpa, Anna Maria [1 ]
Recchimuzzo, Patrizia [1 ]
Dadati, Raffaella [1 ]
Farioli, Renata [2 ]
De Canal, Gabriella [1 ]
Mura, Maria Angela [1 ]
Cesana, Clara [1 ]
机构：
[1] Osped Niguarda Ca Granda, Dept Lab Med, Piazza Osped Maggiore 3, I-20162 Milan, Italy
[2] Osped Niguarda Ca Granda, Dept Hematol, Milan, Italy
来源：
HEMATOLOGICAL ONCOLOGY | 2017年 / 35卷 / 01期
关键词：
acute promyelocytic leukaemia; fluorescence in situ hybridization; microwave oven;
D O I：
10.1002/hon.2194
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Acute promyelocytic leukaemia (APL) is a hematologic malignancy characterized by the rearrangement of the PML and RAR genes, mostly due to a reciprocal chromosomal translocation t(15;17)(q24;q21). A quick APL diagnosis is essential for starting a prompt suitable therapy. We describe a new rapid diagnostic laboratory approach to detect the PML-RAR rearrangement, which gives clear genetic results within 30min of hybridization. It combines quick cell harvesting, fluorescence in situ hybridization performed with commercial DNA probe and microwave beams supplied by a domestic microwave oven. Copyright (c) 2015 John Wiley & Sons, Ltd.
引用
收藏
页码：94 / 100
页数：7
相关论文
共 50 条
  • [41] A PML/RARA chimeric gene on chromosome 12 in a patient with acute promyelocytic leukemia (M4) associated with a new variant translocation: t(12;15;17)(q24;q24;q11)
    Bennour, Ayda
    Tabka, Ikram
    Ben Youssef, Yosra
    Zaier, Monia
    Hizem, Sondess
    Khelif, Abderrahim
    Saad, Ali
    Sennana, Halima
    MEDICAL ONCOLOGY, 2013, 30 (01)
  • [42] A new complex translocation (15;20;17)(q22;p13;q21) in acute promyelocytic leukemia
    Yamamoto, K
    Hamaguchi, H
    Nagata, K
    Kobayashi, M
    Takashima, T
    Taniwaki, M
    CANCER GENETICS AND CYTOGENETICS, 1998, 101 (02) : 89 - 94
  • [43] ISOCHROMOSOME-17Q- IN ACUTE PROMYELOCYTIC LEUKEMIA - MOLECULAR CHARACTERIZATION BY FLUORESCENCE IN-SITU HYBRIDIZATION
    AVENTIN, A
    ESPADALER, M
    MARTINHENAO, GA
    MECUCCI, C
    CANCER GENETICS AND CYTOGENETICS, 1994, 78 (01) : 112 - 113
  • [44] t(5;17)(q13;q24)、t(15;17)(q22;q21)急性早幼粒细胞性白血病1例并文献复习
    孙明
    刘琳
    路明
    山东医药, 2015, 55 (20) : 89 - 90+115
  • [45] Cell lineage specific involvement in acute promyelocytic leukaemia (APL) using a combination of May-Grunwald-Giemsa staining and fluorescence in situ hybridization techniques for the detection of the translocation t(15;17)(q22;q12)
    Haferlach, T
    Löffler, H
    Nickenig, C
    Ramm-Petersen, L
    Meeder, M
    Schoch, R
    Schlegelberger, B
    Schnittger, S
    Schoch, C
    Hiddemann, W
    BRITISH JOURNAL OF HAEMATOLOGY, 1998, 103 (01) : 93 - 99
  • [46] A highly specific and sensitive fluorescence in situ hybridization assay for the detection of t(4;11)(q21;q2 3) and concurrent submicroscopic deletions in acute leukaemias
    König, M
    Reichl, M
    Marschalek, R
    Haas, OA
    Strehl, S
    BRITISH JOURNAL OF HAEMATOLOGY, 2002, 116 (04) : 758 - 764
  • [47] Fluorescence in situ hybridization identifies inversion 16 masked by t(10;16)(q24;q22), t(7;16)(q21;q22), and t(2;16)(q37;q22) in three cases of AML-M4Eo
    Reddy, KS
    Wang, S
    Montgomery, P
    Grove, W
    Robertson, LE
    CANCER GENETICS AND CYTOGENETICS, 2000, 116 (02) : 148 - 152
  • [48] Acute promyelocytic leukemia with the translocation t(15;17)(q22;q21) associated with t(1;2)(q42~43;q11.2~12): A case report
    Wafa A.
    Moassass F.
    Liehr T.
    Al-Ablog A.
    AL-Achkar W.
    Journal of Medical Case Reports, 10 (1)
  • [49] PML/Rara and Rara/PML Chimeric Genes On Derivative Chromosome 17 In a Patient With Acute Promyelocytic Leukemia With Atypical t(15;17)(q11;q21)
    Lopez, Pablo
    Simonet, Solange
    Romanelli, Gerardo
    Infante, Daniela
    Sanguinetti, Analia
    Elizondo, Victoria V.
    Moro, Isabel
    Canessa, Cecilia
    Martinez, Lem
    Escuder, M. Rosario Uriarte
    Bonomi, Rossana
    BLOOD, 2013, 122 (21)
  • [50] Application of interphase fluorescence in situ hybridization for the detection of the Burkitt translocation t(8;14)(q24;q32) in B-cell lymphomas
    Siebert, R
    Matthiesen, P
    Harder, S
    Zhang, YM
    Borowski, A
    Zühlke-Jenisch, R
    Metzke, S
    Joos, S
    Weber-Matthiesen, K
    Grote, W
    Schlegelberger, B
    BLOOD, 1998, 91 (03) : 984 - 990
12345