The utility of multiparametric flow cytometry in the detection of primary effusion lymphoma (PEL)

被引:10
|
作者
Galan, Jacobo [1 ]
Martin, Isaac [1 ]
Carmona, Itziar [1 ]
Maria Rodriguez-Barbero, Jose [2 ]
Cuadrado, Esperanza [3 ]
Garcia-Alonso, Luis [1 ]
Antonio Garcia-Vela, Jose [1 ]
机构
[1] Hosp Univ Getafe, Dept Hematol, Carretera Toledo Km 12 500, Madrid 28905, Spain
[2] Hosp Univ Getafe, Dept Pathol, Madrid, Spain
[3] Hosp Univ Getafe, Dept Biochem, Madrid, Spain
关键词
flow cytometry; extranodal lymphoma; KSHV; HHV8;
D O I
10.1002/cyto.b.21637
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Primary effusion lymphoma (PEL) is a rare B cell lymphoproliferative disorder that arises predominantly in body cavities causing malignant effusions. The incidence of PEL is very low, accounting for approximately 4% of all HIV-associated non-Hodgkin lymphomas. PEL has also been described in elderly patients and after solid-organ transplantation. It is associated in all cases with human herpes virus 8 (HHV8). We describe a case of PEL in a 88-year-old HIV-negative woman who presented with progressive dyspnea and moderate right-sided pleural effusion without significant lymphadenopathies or other effusions. The cytological study of the pleural fluid revealed a dense proliferation of large plasmablastic cells. A six-color multiparametric flow cytometry immunophenotyping study was carried out, and revealed 45% of large in size and high cellular complexity cells positive for CD45 (dim), CD38, CD138, CD30 and HLA-DR; and negative for CD19, CD20, cytoplasmatic CD79a, surface and cytoplasmic light chains Kappa and Lambda, CD3, CD4, CD5, CD7, CD8, CD28, CD56, CD81, and CD117. In situ hybridization for EBV-encoded smalI RNA was negative and immunohistochemistry for Kaposi sarcoma herpesvirus (HHV8) confirmed the diagnosis of PEL. Our results confirm that flow cytometry bring useful data in the diagnosis of large-cell lymphomas involving body cavities. (c) 2018 International Clinical Cytometry Society
引用
收藏
页码:375 / 378
页数:4
相关论文
共 50 条
  • [31] UTILITY OF FLOW-CYTOMETRY IMMUNOPHENOTYPING IN THE FNA DIAGNOSIS OF LYMPHOMA
    ERNER, SM
    ELSHEIKH, TM
    GAGNON, GA
    SILVERMAN, JF
    LABORATORY INVESTIGATION, 1995, 72 (01) : A39 - A39
  • [32] Rapamycin is efficacious against primary effusion lymphoma (PEL) cell lines in vivo by inhibiting autocrine signaling
    Sin, Sang-Hoon
    Roy, Debasmita
    Wang, Ling
    Staudt, Michelle R.
    Fakhari, Farnaz D.
    Patel, Dhavalkumar D.
    Henry, David
    Harrington, William J., Jr.
    Damania, Blossom A.
    Dittmer, Dirk P.
    BLOOD, 2007, 109 (05) : 2165 - 2173
  • [33] Analysis of gene expression in primary effusion lymphoma (PEL): Identification of a protease that degrades extracellular matrix.
    Parker, CJ
    Vreeke, TM
    BLOOD, 2000, 96 (11) : 698A - 698A
  • [34] GO WITH THE FLOW: A RARE CASE OF PRIMARY EFFUSION LYMPHOMA
    Staiano, Peter
    Isaac, Milca
    Oye, Monique
    Alkhasawneh, Ahmad
    Louis, Mariam
    CHEST, 2021, 160 (04) : 1643A - 1643A
  • [35] PRIMARY INTRAOCULAR LYMPHOMA: THE CONTRIBUTION OF FLOW CYTOMETRY TO DIAGNOSIS
    Dilintas, Antonios
    Androulakis, Nikolaos
    Nioti, Eleni
    Spatharaki, Paraskevi
    Papadopoulou, Anastasia
    Nistikaki, Fotini
    Damianaki, Athina
    Kavidopoulou, Aglaia
    Verginis, Panagiotis
    Kalpadaki, Christina
    INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY, 2024, 46 : 32 - 32
  • [36] AIDS-RELATED LYMPHOMA DIAGNOSED BY FLOW-CYTOMETRY OF A PLEURAL EFFUSION
    LEE, AM
    KATNER, HP
    SOUTHERN MEDICAL JOURNAL, 1991, 84 (10) : 1278 - 1279
  • [37] Gene expression profile analysis of AIDS-related primary effusion lymphoma (PEL) suggests a plasmablastic derivation and identifies PEL-specific transcripts
    Klein, U
    Gloghini, A
    Gaidano, G
    Chadburn, A
    Cesarman, E
    Dalla-Favera, R
    Carbone, A
    BLOOD, 2003, 101 (10) : 4115 - 4121
  • [38] Utilizing multiparametric flow cytometry in the diagnosis of patients with primary plasma cell leukemia
    Evans, Laura A.
    Jevremovic, Dragan
    Nandakumar, Bharat
    Dispenzieri, Angela
    Buadi, Francis K.
    Dingli, David
    Lacy, Martha Q.
    Hayman, Suzanne R.
    Kapoor, Prashant
    Leung, Nelson
    Fonder, Amie
    Hobbs, Miriam
    Hwa, Yi Lisa
    Muchtar, Eli
    Warsame, Rahma
    Kourelis, Taxiarchis V.
    Go, Ronald
    Russell, Stephen
    Lust, John A.
    Lin, Yi
    Siddiqui, Mustaqeem
    Kyle, Robert A.
    Gertz, Morie A.
    Rajkumar, S. Vincent
    Kumar, Shaji K.
    Gonsalves, Wilson I.
    AMERICAN JOURNAL OF HEMATOLOGY, 2020, 95 (06) : 637 - 642
  • [39] Primary effusion lymphoma
    Aozasa, K
    INTERNAL MEDICINE, 2004, 43 (03) : 171 - 171
  • [40] Primary effusion lymphoma
    Choi, Sarah M.
    Chen, Yi-Hua
    BLOOD, 2015, 126 (22) : 2522 - 2522