Detection of Cytolethal Distending Toxin and Other Virulence Characteristics of Enteropathogenic Escherichia coli Isolates from Diarrheal Patients in Republic of Korea

被引:11
|
作者
Kim, Jong-Hyun [1 ]
Kim, Jong-Chul [1 ]
Choo, Yun-Ae [1 ]
Jang, Hyun-Chul [1 ]
Choi, Yeon-Hwa [1 ]
Chung, Jae-Keun [2 ]
Cho, Seung-Hak [1 ]
Park, Mi-Seon [1 ]
Lee, Bok-Kwon [1 ]
机构
[1] NIH, Div Enter Bacterial Infect, Ctr Infect Dis, Seoul 122701, South Korea
[2] Gwang Ju Inst Hlth & Environm, Kwangju 502240, South Korea
关键词
Cytolethal distending toxin; atypical EPEC; typical EPEC; bundle-forming pilus; BUNDLE-FORMING PILUS; CDT; STRAINS; CLONING; GENES; IDENTIFICATION; EXPRESSION; CYTOTOXIN; SHIGELLA;
D O I
10.4014/jmb.0801.033
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Cytolethal distending toxins (CDTs) represent an emerging family of newly described bacterial products that are produced by a number of pathogens. The genes encoding these toxins have been identified as a cluster of three adjacent genes, cdtA, cdtB, and cdtC plus 5 cdt genetic variants, designated as cdt-I, cdt-II, cdt-III, cdt-IV, and cdt-V, have been identified to date. In this study, a general multiplex PCR system designed to detect Escherichia coli wits was applied to investigate the presence of cdt genes among isolates. As a result, among 366 E. coli strains, 2.7% were found to carry the cdtB gene. In addition, the use of type-specific primers revealed the presence of cdt-I, cdtIV, and cdt-V types of the cdt gene, yet no cdt-II or cdt-III strains. The presence of other virulence genes (stx1, stx2, eae, bjp, espA, espB, and espD) was also investigated using a PCR assay. Among the 10 cdtB gene-positive strains, 8 were identified as CDT-producing typical enteropathogenic E. coli (EPEC) strains (eae(+), bfp(+)), whereas 2 were identified as CDT-producing atypical EPEC strains (eae(+), bfp(-)). When comparing the cytotoxic activity of the CDT-producing typical and atypical SPEC strains, the CDT-producing atypical EPEC strains appeared to be less toxic than the CDT-producing typical SPEC strains.
引用
收藏
页码:525 / 529
页数:5
相关论文
共 50 条
  • [21] An inducible lambdoid prophage encoding cytolethal distending toxin (Cdt-I) and a type III effector protein in enteropathogenic Escherichia coli
    Asakura, Masahiro
    Hinenoya, Atsushi
    Alam, Mohammad S.
    Shima, Kensuke
    Zahid, Shamim Hasan
    Shi, Lei
    Sugimoto, Norihiko
    Ghosh, A. N.
    Ramamurthy, T.
    Faruque, Shah M.
    Nair, G. Balakrish
    Yamasaki, Shinji
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (36) : 14483 - 14488
  • [22] Occurrence, serotypes and virulence characteristics of Shiga toxin-producing and Enteropathogenic Escherichia coli isolates from dairy cattle in South Africa
    Olawole, Alaba S.
    Malahlela, Mogaugedi N.
    Fonkui, Thierry Y.
    Marufu, Munyaradzi C.
    Cenci-Goga, Beniamino T.
    Grispoldi, Luca
    Etter, Eric M. C.
    Tagwireyi, Whatmore M.
    Karama, Musafiri
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2024, 40 (10):
  • [23] Cytolethal distending toxin (Cdt)-producing Escherichia coli isolated from a child with bloody diarrhea in Japan
    Hinenoya, Atsushi
    Nagita, Akira
    Asakura, Masahiro
    Tsukamoto, Teizo
    Ramamuthy, Thandavarayan
    Nair, Gopinath Balakrish
    Takeda, Yoshifumi
    Yamasaki, Shinji
    MICROBIOLOGY AND IMMUNOLOGY, 2007, 51 (04) : 435 - 438
  • [24] Genetic and virulence characteristics of hybrid Shiga toxin-producing and atypical enteropathogenic Escherichia coli strains isolated in South Korea
    Lee, Woojung
    Ha, Jina
    Choi, Jaehyun
    Jung, Yewon
    Kim, Eiseul
    An, Eun Sook
    Kim, Seung Hwan
    Shin, Hakdong
    Ryu, Sangryeol
    Kim, Soon Han
    Kim, Hae-Yeong
    FRONTIERS IN MICROBIOLOGY, 2024, 15
  • [25] Characteristics of cytotoxic necrotizing factor and cytolethal distending toxin producing Escherichia coli strains isolated from meat samples in Northern Ireland
    Kadhum, HJ
    Ball, HJ
    Oswald, E
    Rowe, MT
    FOOD MICROBIOLOGY, 2006, 23 (05) : 491 - 497
  • [26] ENTEROTOXIGENICITY OF ENTEROPATHOGENIC ESCHERICHIA-COLI ISOLATED FROM SPORADIC DIARRHEAL PATIENTS
    RAMACHANDRAN, VG
    VARGHESE, A
    INDIAN JOURNAL OF MEDICAL RESEARCH, 1984, 80 (NOV) : 505 - 511
  • [27] Virulence Genes of Escherichia coli Isolates from Piglets with Diarrhea in Korea
    Chae, Myeong Ju
    Cho, Jae Keun
    Lee, Young Ju
    JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2012, 11 (01): : 9 - 12
  • [28] Detection of cytolethal distending toxin activity and cdt genes in Actinobacillus actinomycetemcomitans isolates from geographically diverse populations
    Fabris, AS
    DiRienzo, JM
    Wïkstrom, M
    Mayer, MPA
    ORAL MICROBIOLOGY AND IMMUNOLOGY, 2002, 17 (04): : 231 - 238
  • [29] Prevalence of virulence genes and cytolethal distending toxin production in Campylobacter jejuni isolated from diarrhoeal patients in Bangladesh
    Talukder, K. A.
    Aslam, M.
    Islam, Z.
    Azmi, I. J.
    Hossain, S.
    Endtz, H. P.
    Nair, G.
    Sack, D. A.
    ZOONOSES AND PUBLIC HEALTH, 2007, 54 : 105 - 105
  • [30] Detection of Integrons from Escherichia coli Isolates Obtained from Some Selected Animals in Republic of Korea
    Mehedi Mahmudul Hasan
    Shiuly Rahman Tinni
    Indian Journal of Microbiology, 2011, 51 : 144 - 146