Sensitive analysis of protein phosphatase inhibitors by the firefly bioluminescence system: Application to PP1 gamma

被引：9

作者：

Sugiyama, Y

Fujimoto, K

Ohtani, II

Takai, A

Isobe, M

机构：

[1] NAGOYA UNIV, ORGAN CHEM LAB, SCH AGR SCI, NAGOYA, AICHI 46401, JAPAN

[2] NAGOYA UNIV, SCH MED, DEPT PHYSIOL, NAGOYA, AICHI 466, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 08期

关键词：

protein phosphatase inhibitors; bioluminescence; PP1; gamma; tautomycin;

D O I：

10.1271/bbb.60.1260

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein phosphatases are classified into types 1 (PP1) and 2 (PP2A, 2B, and 2C). We have already established a new analysis method for PP2A inhibitors by using the firefly bioluminescence system for detection. This method was successfully applied to determine the PP1 gamma inhibitory activity of known inhibitors, i.e., calyculin A, microcystin-LR, and tautomycin, requiring less than 10 pmol of a sample.

引用

页码：1260 / 1264

页数：5

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