Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay

被引：10
|
作者：
Burkhalter, Kristen L. [1 ]
Savage, Harry M. [1 ]
机构：
[1] Ctr Dis Control & Prevent, 3156 Rampart Rd, Ft Collins, CO 80521 USA
来源：
EMERGING INFECTIOUS DISEASES | 2017年 / 23卷 / 04期
关键词：
VENEZUELAN EQUINE ENCEPHALITIS; HOLDING CONDITIONS; WEST-NILE; POOLS; RNA;
D O I：
10.3201/eid2304.161772
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We assayed Zika virus-infected mosquitoes stored at room temperature for <= 30 days for live virus by using plaque assay and virus RNA by using real-time reverse transcription PCR. Viable virus was detected in samples stored <= 10 days, and virus RNA was detected in samples held for 30 days.
引用
收藏
页码：680 / 681
页数：2
相关论文
共 50 条
  • [41] Real-time RT-PCR Assay for the detection of Tahyna Virus
    Li Hao
    Cao Yu Xi
    He Xiao Xia
    Fu Shi Hong
    Lyu Zhi
    He Ying
    Gao Xiao Yang
    Liang Guo Dong
    Wang Huang Yu
    BIOMEDICAL AND ENVIRONMENTAL SCIENCES, 2015, 28 (05) : 374 - 377
  • [42] Real-time RT-PCR Assay for the detection of Tahyna Virus
    LI Hao
    CAO Yu Xi
    HE Xiao Xia
    FU Shi Hong
    LYU Zhi
    HE Ying
    GAO Xiao Yan
    LIANG Guo Dong
    WANG Huan Yu
    Biomedical and Environmental Sciences, 2015, 28 (05) : 374 - 377
  • [43] Development of real-time PCR assay for specific detection of cowpox virus
    Gavrilova, Elena V.
    Shcherbakov, Dmitrii N.
    Maksyutov, Rinat A.
    Shchelkunov, Sergei N.
    JOURNAL OF CLINICAL VIROLOGY, 2010, 49 (01) : 37 - 40
  • [44] Development of a Real-Time PCR Assay for the Detection of the Phlebovirus Fermo Virus
    Calzolari, Mattia
    Russo, Simone
    Marzani, Katia
    Dalmonte, Gastone
    Ricchi, Matteo
    Bonilauri, Paolo
    VIRUSES-BASEL, 2023, 15 (10):
  • [45] Rapid preclinical detection of Sheeppox virus by a real-time PCR assay
    Balinsky, C. A.
    Delhon, G.
    Smoliga, G.
    Prarat, M.
    French, R. A.
    Geary, S. J.
    Rock, D. L.
    Rodriguez, L. L.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (02) : 438 - 442
  • [46] Quantitative detection of influenza virus by real-time RT-PCR to measure protective efficacy of the live vaccine: a comparison of quantitative real-time PCR and plaque assay
    Chung, YM
    Chun, HO
    Kim, YG
    Kim, SW
    Kim, HA
    Kim, YH
    Ha, SH
    Chae, MY
    Chang, JH
    Kim, JH
    FASEB JOURNAL, 2005, 19 (04): : A897 - A897
  • [47] Fast track diagnostics Zika virus kit - A real-time PCR based diagnostics assay
    Leidinger-Kaufmann, P.
    Steimer, M.
    Carman, W.
    INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2016, 53 : 109 - 109
  • [48] Quantitative detection of Citrus tristeza virus in citrus and aphids by real-time reverse transcription-PCR (TaqMan®)
    Saponari, Maria
    Manjunath, Keremane
    Yokomi, Raymond K.
    JOURNAL OF VIROLOGICAL METHODS, 2008, 147 (01) : 43 - 53
  • [49] Development of a real-time reverse-transcription PCR for detection of Newcastle disease virus RNA in clinical samples
    Wise, MG
    Suarez, DL
    Seal, BS
    Pedersen, JC
    Senne, DA
    King, DJ
    Kapczynski, DR
    Spackman, E
    JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (01) : 329 - 338
  • [50] Detection and quantification of canine influenza virus by one-step real-time reverse transcription PCR.
    Spindel, M. E.
    Lunn, K. F.
    Dillion, S.
    Landolt, G. A.
    JOURNAL OF VETERINARY INTERNAL MEDICINE, 2007, 21 (03) : 576 - 576
12345