Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay

被引：10
|
作者：
Burkhalter, Kristen L. [1 ]
Savage, Harry M. [1 ]
机构：
[1] Ctr Dis Control & Prevent, 3156 Rampart Rd, Ft Collins, CO 80521 USA
来源：
EMERGING INFECTIOUS DISEASES | 2017年 / 23卷 / 04期
关键词：
VENEZUELAN EQUINE ENCEPHALITIS; HOLDING CONDITIONS; WEST-NILE; POOLS; RNA;
D O I：
10.3201/eid2304.161772
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We assayed Zika virus-infected mosquitoes stored at room temperature for <= 30 days for live virus by using plaque assay and virus RNA by using real-time reverse transcription PCR. Viable virus was detected in samples stored <= 10 days, and virus RNA was detected in samples held for 30 days.
引用
收藏
页码：680 / 681
页数：2
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