GERMINATION AND SUBSEQUENT INACTIVATION OF BACILLUS SUBTILIS SPORES BY PULSED ELECTRIC FIELD TREATMENT

被引：15

作者：

Shin, J. K. ^{[2
]}

Lee, S. J. ^{[3
]}

Cho, H. Y. ^{[4
]}

Pyun, Y. R. ^{[3
]}

Lee, J. H. ^{[1
]}

Chung, M. S. ^{[1
]}

机构：

[1] Ewha Womans Univ, Dept Food Sci & Technol, Seoul 120750, South Korea

[2] Jeonju Univ, Dept Korean Tradit Food Culture, Jeonju 560759, South Korea

[3] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea

[4] CHO Korea, Puchon 421741, South Korea

来源：

JOURNAL OF FOOD PROCESSING AND PRESERVATION | 2010年 / 34卷 / 01期

关键词：

BACTERIAL-SPORES; SACCHAROMYCES-CEREVISIAE; TEMPERATURE; OUTGROWTH; MICROORGANISMS; INHIBITION; FOODS;

D O I：

10.1111/j.1745-4549.2008.00321.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The use of high-intensity pulsed electric field (PEF) as a nonthermal method for triggering spore germination was investigated. Spores suspended in 0.01% NaCl solution (ca. 10(8) cells/mL) were treated with an exponential-decay pulse (20-50 kV/cm) at selected temperatures. The inactivation resulting from the direct effect of PEF treatment was less than 0.5 log cycles, even with a relatively high electric field intensity of 40 kV/cm. Most of the surviving PEF-treated spores germinated after inoculation into selected germination media (0.85% NaCl solution, nutrient broth [NB], or tryptic soy broth [TSB]) and subsequent incubation at 37C for 40 min. The extent of germination increased with the treatment temperature and electric field intensity. More than 98% of surviving spores in TSB germinated during incubation after PEF treatment with an electric field strength of 40 kV/cm for 1,000 mu s at 50C. The rate of germination in nutrient-rich TSB medium was higher than that in NB.

引用

页码：43 / 54

页数：12

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