Preparation of urease-immobilized polymeric membranes and their function

被引：8
|
作者：
Uragami, T. [1 ]
Ueguchi, K.
Watanabe, A.
Miyata, T.
机构：
[1] Kansai Univ, Unite Chem, Fac Engn, Suita, Osaka 5648680, Japan
[2] Kansai Univ, High Technol Res Ctr, Suita, Osaka 5648680, Japan
来源：
CATALYSIS TODAY | 2006年 / 118卷 / 1-2期
关键词：
enzyme; immobilization; copolymerization of vinylized urease; polyion complex; hydrolysis of urea; ultrafiltration; Michaelis-Menten kinetics;
D O I：
10.1016/j.cattod.2005.12.007
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Two kinds of urease-immobilized polymer membranes were prepared. One was prepared by bulk-copolymerizing a mixture consisting of vinylized urease (VU), acrylamide (AAm), 2-hydroxylethylmethacrylate (HEMA) and a cross-linker (urease-immobilized poly(VU-AAm-HEMA) membrane) and another was prepared by ultrafiltrating a mixture composed of urease, quaternized chitosan and sodium carboxymethylcellulose in an aqueous sodium bromide solution (urease-immobilized polyion complex membrane). The permeation and hydrolytic characteristics of aqueous urea solutions were kinetically investigated under various conditions through urease-immobilized membranes. The hydrolysis of urea through urease-immobilized membranes followed Michaelis-Menten kinetics and is discussed herein. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：158 / 165
页数：8
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