Urinary proteins detected using modern proteomics intervene in early type 2 diabetic kidney disease - a pilot study

被引：9
|
作者：
Golea-Secara, Alina [1 ,2 ]
Munteanu, Cristian [3 ]
Sarbu, Mirela [4 ]
Cretu, Octavian M. [2 ,5 ]
Velciov, Silvia [1 ,2 ]
Vlad, Adrian [2 ,6 ]
Bob, Flaviu [1 ,2 ]
Gadalean, Florica [1 ,2 ]
Gluhovschi, Cristina [2 ]
Milas, Oana [1 ,2 ]
Simulescu, Anca [1 ,2 ]
Mogos-Stefan, Maria [1 ,2 ]
Patruica, Mihaela [1 ,2 ]
Petrica, Ligia [1 ,2 ,7 ]
Zamfir, Alina D. [4 ]
机构：
[1] Cty Emergency Hosp Timisoara, Dept Nephrol, Timisoara, Romania
[2] Victor Babes Univ Med & Pharm, Timisoara, Romania
[3] Inst Biochem, Dept Bioinformat & Struct Biochem, Bucharest, Romania
[4] Natl Inst Res & Dev Electrochem & Condensed Matte, Timisoara, Romania
[5] Municipal Emergency Hosp Timisoara, Dept Surg 1, Timisoara, Romania
[6] Cty Emergency Hosp, Dept Diabet & Metab Dis, Timisoara, Romania
[7] Victor Babes Univ Med & Pharm, Ctr Translat Res & Syst Med, Timisoara, Romania
来源：
BIOMARKERS IN MEDICINE | 2020年 / 14卷 / 16期
关键词：
albuminuria; diabetes mellitus Type 2; inflammation; proximal tubule dysfunction; urinary proteomics; BIOMARKERS; IDENTIFICATION; NEPHROPATHY; PATHOPHYSIOLOGY; EXPRESSION; PEPTIDES;
D O I：
10.2217/bmm-2020-0308
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Aim:An advanced proteomics platform for protein biomarker discovery in diabetic chronic kidney disease (DKD) was developed, validated and implemented.Materials & methods:Three Type 2 diabetes mellitus patients and three control subjects were enrolled. Urinary peptides were extracted, samples were analyzed on a hybrid LTQ-Orbitrap Velos Pro instrument. Raw data were searched using the SEQUEST algorithm and integrated into Proteome Discoverer platform.Results & discussion:Unique peptide sequences, resulted sequence coverage, scoring of peptide spectrum matches were reported to albuminuria and databases. Five proteins that can be associated with early DKD were found: apolipoprotein AI, neutrophil gelatinase-associated lipocalin, cytidine deaminase, S100-A8 and hemoglobin subunit delta.Conclusion:Urinary proteome analysis could be used to evaluate mechanisms of pathogenesis of DKD.
引用
收藏
页码：1521 / 1537
页数：17
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