A sensitive and selective turn-on fluorescent probe for hypochlorous acid based on a thiorhodamine 6G amide, and its application in cellular imaging

被引：19

作者：

Hu, Zhi-Qiang ^{[1
]}

Zhu, Jiang-Hua ^{[1
]}

Gu, Ying-Ying ^{[1
]}

Hu, Wen-Zhou ^{[1
]}

Li, Ming ^{[1
]}

Jiang, Yi ^{[1
]}

机构：

[1] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Peoples R China

来源：

MICROCHIMICA ACTA | 2014年 / 181卷 / 11-12期

基金：

中国国家自然科学基金;

关键词：

Fluorescent chemodosimeter; Rhodamine-6G derivative; HClO; Image; LIVING CELLS; HG2+ IONS; RHODAMINE; CHEMOSENSOR; FRET; RECEPTOR;

D O I：

10.1007/s00604-014-1280-z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report that a previously found fluorescent chemodosimeter for Hg(II) ions can also be used as a fluorescent probe for hypochlorous acid (HClO) in that its fluorescence intensity (excited at 520 nm and measured at 563 nm) is linearly proportional to the concentration of HClO in the 1-100 mu M range and with a 0.1 mu M detection limit. The probe can detect HClO with virtually no interference by Hg(II) and other metal ions. The mechanism for the high selectivity for HClO has been studied. The probe was applied to image hypochlorite in E. coli cells.

引用

页码：1401 / 1406

页数：6

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