Native Mass Spectrometry of Recombinant Proteins from Crude Cell Lysates

被引：41

作者：

Gan, Jinrui ^{[1
]}

Ben-Nissan, Gili ^{[1
]}

Arkind, Galina ^{[1
]}

Tarnavsky, Mark ^{[1
]}

Trudeau, Devin ^{[1
]}

Garcia, Lianet Noda ^{[1
]}

Tawfik, Dan S. ^{[1
]}

Sharon, Michal ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Biomol Sci, IL-7610001 Rehovot, Israel

来源：

ANALYTICAL CHEMISTRY | 2017年 / 89卷 / 08期

基金：

欧洲研究理事会;

关键词：

STRUCTURAL-CHARACTERIZATION; PHARMACEUTICALS; EXPRESSION; BIOLOGY;

D O I：

10.1021/acs.analchem.7b00398

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Determining the properties of proteins prior to purification saves time and labor. Here, we demonstrate a native mass spectrometry approach for rapid characterization of overexpressed proteins directly in crude cell lysates. The method provides immediate information on the identity, solubility, oligomeric state, overall structure, and stability, as well as ligand binding, without the need for purification.

引用

页码：4398 / 4404

页数：7

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