Purification and characterization of a rhamnose-binding lectin with immunoenhancing activity from grass carp (Ctenopharyngodon idellus) ovaries

被引：42
|
作者：
Lam, YW [1 ]
Ng, TB [1 ]
机构：
[1] Chinese Univ Hong Kong, Dept Biochem, Fac Med, Shatin, Hong Kong, Peoples R China
来源：
PROTEIN EXPRESSION AND PURIFICATION | 2002年 / 26卷 / 03期
关键词：
grass carp; lectin; rhamnose-binding;
D O I：
10.1016/S1046-5928(02)00559-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rhamnose-specific lectin was isolated from ovaries of the grass carp (Ctenopharyngodon idellus). The grass carp lectin possesses a molecular mass of 205 kDa. It is composed of six subunits each with a molecular mass of 35 kDa. The N-terminal amino acid sequence of the grass carp shows similarity to those of other fish species with 26-35% amino acid identity. It is mitogenic toward murine splenocytes and peritoneal exudate cells. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：378 / 385
页数：8
相关论文
共 50 条
  • [1] The immunostimulatory activity and stability of grass carp (Ctenopharyngodon idellus) roe lectin
    Ng, TB
    Lam, YW
    Woo, NYS
    VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2003, 94 (3-4) : 105 - 112
  • [2] Affinity Chromatographic Purification and Enzymatic Characterization of Trypsin from Grass Carp(Ctenopharyngodon idellus)
    Li C.
    Gao L.
    Cui X.
    Han Y.
    Zhu D.
    Li J.
    Shipin Kexue/Food Science, 2021, 42 (10): : 178 - 183
  • [3] Purification and characterization of a gelatinolytic matrix metalloproteinase from the skeletal muscle of grass carp (Ctenopharyngodon idellus)
    Wu, Jiu-Lin
    Ge, Shang-Ying
    Cai, Zhi-Xing
    Liu, Hui
    Liu, Ying-Xiang
    Wang, Jian-Hua
    Zhang, Qi-Qing
    FOOD CHEMISTRY, 2014, 145 : 632 - 638
  • [4] Construction and Characterization of Grass Carp (Ctenopharyngodon idellus) Fosmid Library
    JIA Zhen-hu1
    Agricultural Sciences in China, 2010, 9 (09) : 1347 - 1352
  • [5] Purification and characterization of α2-macroglobulin from grass carp Ctenopharyngodon idellus:: Cloning a segment of the corresponding gene
    Li, FL
    Lu, CP
    FISH & SHELLFISH IMMUNOLOGY, 2006, 20 (04) : 474 - 481
  • [6] GENERATION AND ANALYSIS OF ESTs FROM THE GRASS CARP, CTENOPHARYNGODON IDELLUS
    Xu, Bing
    Wang, Shaolin
    Jiang, Yan
    Yang, Linlin
    Li, Ping
    Xie, Caixia
    Xing, Jubin
    Ke, Zhonghe
    Li, Jiale
    Gai, Junwei
    Yang, Guimei
    Bao, Baolong
    Liu, Zhanjiang
    ANIMAL BIOTECHNOLOGY, 2010, 21 (04) : 217 - 225
  • [7] Isolation and genetic characterization of Flavobacterium columnare from grass carp, Ctenopharyngodon idellus, in China
    Lu, Zijun
    Gao, Ren
    Duan, Yafei
    Han, Rui
    Guo, Wenjie
    Dan, Xueming
    Li, Yanwei
    AQUACULTURE, 2021, 541
  • [8] An acidic protease from the grass carp intestine (Ctenopharyngodon idellus)
    Liu, Zhong-yi
    Wang, Zhang
    Zhang, Han
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2008, 149 (01): : 83 - 90
  • [9] Isolation and characterization of Citrobacter spp. from the intestine of grass carp Ctenopharyngodon idellus
    Lue, Aijun
    Hu, Xiucai
    Zheng, Lu
    Zhu, Aihua
    Cao, Chengliang
    Jiang, Jihong
    AQUACULTURE, 2011, 313 (1-4) : 156 - 160
  • [10] PARTIAL CHARACTERIZATION OF GELATINOLYTIC PROTEINASES FROM THE SKELETAL MUSCLE OF GRASS CARP (CTENOPHARYNGODON IDELLUS)
    Wu, Jiu-Lin
    Chen, Shan-Fei
    Cai, Zhi-Xing
    Chen, Li
    Cao, Min-Jie
    Zhang, Qi-Qing
    JOURNAL OF FOOD BIOCHEMISTRY, 2012, 36 (06) : 648 - 656
12345