GENERATION AND ANALYSIS OF ESTs FROM THE GRASS CARP, CTENOPHARYNGODON IDELLUS

被引：5

作者：

Xu, Bing ^{[1
]}

Wang, Shaolin ^{[2
,3
]}

Jiang, Yan ^{[1
]}

Yang, Linlin ^{[1
]}

Li, Ping ^{[2
,3
]}

Xie, Caixia ^{[1
]}

Xing, Jubin ^{[1
]}

Ke, Zhonghe ^{[1
]}

Li, Jiale ^{[1
]}

Gai, Junwei ^{[1
]}

Yang, Guimei ^{[1
]}

Bao, Baolong ^{[1
]}

Liu, Zhanjiang ^{[1
,2
,3
]}

机构：

[1] Shanghai Ocean Univ, Minist Educ, Key Lab Explorat & Utilizat Aquat Genet Resources, Shanghai 201306, Peoples R China

[2] Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA

[3] Auburn Univ, Aquat Genom Unit, Program Cell & Mol Biosci, Auburn, AL 36849 USA

来源：

ANIMAL BIOTECHNOLOGY | 2010年 / 21卷 / 04期

基金：

中国国家自然科学基金;

关键词：

EST; Genome; Grass carp; Marker; Microsatellite; SNP; CATFISH ICTALURUS-PUNCTATUS; TRANSCRIPTOME ANALYSIS; INITIAL ANALYSIS; GENE; EXPRESSION; RESISTANCE; GENOMICS; TOOL;

D O I：

10.1080/10495398.2010.505843

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Grass carp, Ctenopharyngodon idellus (Valenciennes, 1844), is an economically important species widely cultured in the world, but its genome research resources are largely lacking. The objectives of this study were to construct normalized cDNA libraries for efficient EST analysis, to generate ESTs from these libraries, and to identify EST-related molecular markers such as microsatellites and single nucleotide polymorphisms (SNPs) for genetic analysis of this species. A total of 6,269 ESTs were generated representing 4,815 unique sequences, from which 105 putative microsatellites and 5,228 SNPs were identified. These genome resources provide the material basis for future genetic and functional analyses in this species.

引用

页码：217 / 225

页数：9

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