Multiple chromosomal banding in grass carp, Ctenopharyngodon idellus

When Giemsa-stained metaphases of the grass carp Ctenopharyngodon idellus were digested with trypsin and then restained with basic Giemsa staining solution, chromosomes showed highly reproducible structural bands. The banding pattern was chromosome–specific, and corresponding chromosomes in different metaphases were identified unequivocally. A complete banding pattern of the fish with 217 bands was constructed. These results strongly indicate that good quality structural banding patterns can be obtained from metaphase chromosomes in fish.