Hyperoxia is not an essential condition for status epilepticus induced cell death in organotypic hippocampal slice cultures

被引：9
|
作者：
Pomper, JK [1 ]
Hoffmann, U [1 ]
Kovács, R [1 ]
Gabriel, S [1 ]
Heinemann, U [1 ]
机构：
[1] Humboldt Univ, Univ Hosp Charite, Johannes Muller Inst Physiol, D-10117 Berlin, Germany
来源：
EPILEPSY RESEARCH | 2004年 / 59卷 / 01期
关键词：
seizure; spreading depression; high oxygen; ROS; cell death; propidum iodide;
D O I：
10.1016/j.eplepsyres.2004.03.002
中图分类号：
R74 [神经病学与精神病学];
学科分类号：
摘要：
The low Mg2+ model of epilepsy in organotypic hippocampal slice Cultures is used to elucidate the mechanism underlying neuronal cell death following sustained epileptiform activity. However, the high oxygen tension of 95% widely used in this model is capable of inducing neuronal cell death by itself. Here we demonstrate that even under normoxic conditions 1h of epileptiform activity induced neuronal cell death as assessed by Propidium Iodide uptake. We conclude that hyperoxia is not essential for status epilepticus induced neuronal cell death in this model. (C) 2004 Elsevier B.V.. All rights reserved.
引用
收藏
页码：61 / 65
页数：5
相关论文
共 50 条
  • [41] LITHIUM PREVENTS EXCITOTOXIC CELL DEATH OF MOTONEURONS IN ORGANOTYPIC SLICE CULTURES OF SPINAL CORD
    Caldero, J.
    Brunet, N.
    Tarabal, O.
    Piedrafita, L.
    Hereu, M.
    Ayala, V.
    Esquerda, J. E.
    NEUROSCIENCE, 2010, 165 (04) : 1353 - 1369
  • [42] Effects of the Hybridization of Opioid and Neurotensin Pharmacophores on Cell Survival in Rat Organotypic Hippocampal Slice Cultures
    Kleczkowska, Patrycja
    Kawalec, Maria
    Bujalska-Zadrozny, Magdalena
    Filip, Magorzata
    Zablocka, Barbara
    Lipkowski, Andrzej W.
    NEUROTOXICITY RESEARCH, 2015, 28 (04) : 352 - 360
  • [43] Effects of the Hybridization of Opioid and Neurotensin Pharmacophores on Cell Survival in Rat Organotypic Hippocampal Slice Cultures
    Patrycja Kleczkowska
    Maria Kawalec
    Magdalena Bujalska-Zadrozny
    Małgorzata Filip
    Barbara Zablocka
    Andrzej W. Lipkowski
    Neurotoxicity Research, 2015, 28 : 352 - 360
  • [44] Effects of α7 Nicotinic Receptor Activation on Cell Survival in Rat Organotypic Hippocampal Slice Cultures
    Denise F. Happ
    R. Andrew Tasker
    Neurotoxicity Research, 2018, 33 : 887 - 895
  • [45] Basal forebrain cholinergic cell attachment and neurite outgrowth on organotypic slice cultures of hippocampal formation
    Tsai, ES
    Haraldson, SJ
    Baratta, J
    Lander, AD
    Yu, J
    Robertson, RT
    NEUROSCIENCE, 2002, 115 (03) : 815 - 827
  • [46] Effects of α7 Nicotinic Receptor Activation on Cell Survival in Rat Organotypic Hippocampal Slice Cultures
    Happ, Denise F.
    Tasker, R. Andrew
    NEUROTOXICITY RESEARCH, 2018, 33 (04) : 887 - 895
  • [47] LOCALIZED GENE-TRANSFER INTO ORGANOTYPIC HIPPOCAMPAL SLICE CULTURES AND ACUTE HIPPOCAMPAL SLICES
    CASACCIABONNEFIL, P
    BENEDIKZ, E
    SHEN, H
    STELZER, A
    EDELSTEIN, D
    GESCHWIND, M
    BROWNLEE, M
    FEDEROFF, HJ
    BERGOLD, PJ
    JOURNAL OF NEUROSCIENCE METHODS, 1993, 50 (03) : 341 - 351
  • [48] The Analysis of Neurovascular Remodeling in Entorhino-hippocampal Organotypic Slice Cultures
    Chip, Sophorn
    Zhu, Xinzhou
    Kapfhammer, Josef P.
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2014, (92):
  • [49] Analyses of neuronal damage in excitotoxically lesioned organotypic hippocampal slice cultures
    Ebrahimi, Fahim
    Hezel, Marcus
    Koch, Marco
    Ghadban, Chalid
    Korf, Horst-Werner
    Dehghani, Faramarz
    ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER, 2010, 192 (04) : 199 - 204
  • [50] Microglial polarization and plasticity: Evidence from organotypic hippocampal slice cultures
    Ajmone-Cat, Maria Antonietta
    Mancini, Melissa
    De Simone, Roberta
    Cilli, Piera
    Minghetti, Luisa
    GLIA, 2013, 61 (10) : 1698 - 1711
12345