Hyperoxia is not an essential condition for status epilepticus induced cell death in organotypic hippocampal slice cultures

被引：9
|
作者：
Pomper, JK [1 ]
Hoffmann, U [1 ]
Kovács, R [1 ]
Gabriel, S [1 ]
Heinemann, U [1 ]
机构：
[1] Humboldt Univ, Univ Hosp Charite, Johannes Muller Inst Physiol, D-10117 Berlin, Germany
来源：
EPILEPSY RESEARCH | 2004年 / 59卷 / 01期
关键词：
seizure; spreading depression; high oxygen; ROS; cell death; propidum iodide;
D O I：
10.1016/j.eplepsyres.2004.03.002
中图分类号：
R74 [神经病学与精神病学];
学科分类号：
摘要：
The low Mg2+ model of epilepsy in organotypic hippocampal slice Cultures is used to elucidate the mechanism underlying neuronal cell death following sustained epileptiform activity. However, the high oxygen tension of 95% widely used in this model is capable of inducing neuronal cell death by itself. Here we demonstrate that even under normoxic conditions 1h of epileptiform activity induced neuronal cell death as assessed by Propidium Iodide uptake. We conclude that hyperoxia is not essential for status epilepticus induced neuronal cell death in this model. (C) 2004 Elsevier B.V.. All rights reserved.
引用
收藏
页码：61 / 65
页数：5
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