Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain

被引：3
|
作者：
Zheng Haixue
Liu Xinagtao [1 ]
Shang Youjun
Wu Jinyan
Bai Xingwen
Jin Ye
Sun Shiqi
Guo Huichen
Tian Hong
Feng Xia
Yin Shaunghui
Guo Jianhong
Cong Guozheng
Liu Zaixin
Chang Huiyun
Ma Junwu
Xie Qingge
机构：
[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Minist Agr,Key Lab Anim Virol, Lanzhou 730046, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100081, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2006年 / 51卷 / 17期
关键词：
swine vesicular disease virus; full-length cDNA; infectious clone; pSVOK(12); reverse genetics;
D O I：
10.1007/s11434-006-2095-z
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK(12) was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In vitro transcription RNA from pSVOK(12) transfected IBRS-2 cells and the recovered virus RNA were isolated and sequenced, then indirect hemagglutination test, indirect immunofluorescence assays, eleectron microscope test, 50% tissue culture infecting dose (TCID50) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. The result showed that the infectious clones we obtained and the virus derived from pSVOK(12) had the same biological properties as the parental strain HK/70. The full-length infectious cDNA clone, pSVOK(12), will be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and replication of SVDV.
引用
收藏
页码：2072 / 2078
页数：7
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