Ice formation of vitrification solutions for cryopreservation of tissues

被引：2
|
作者：
Liu, B. L. [1 ]
McGrath, J. J. [1 ]
机构：
[1] Shanghai Univ Sci & Technol, Inst Cryomed, Shanghai 200093, Peoples R China
来源：
2005 27TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-7 | 2005年
关键词：
vitrification; ice quantity; cryopreservation;
D O I：
10.1109/IEMBS.2005.1616247
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Based on Boutron's semi-empirical crystalization theory, a quantitative microscopic imaging method was developed to determine the quantity of ice in thin films of vitrification solutions. A universal equation was obtained for various vitrification solutions. The new method was applied to determine the ice quantity and critical cooling rates for two new vitrification solutions. This new method has several advantages including the fact that it is cheaper than differential scanning calorimetry (DSC) and that it can be used to study vitrification solutions with critical cooling rates extending beyond the maximum cooling rate of a typical DSC system.
引用
收藏
页码：7501 / 7504
页数：4
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